秸秆类生物质酶解动力学与光合生物产氢特性研究

采用类分形动力学对4种常见秸秆类生物质的酶水解过程及其光合生物产氢能力进行了实验研究,并分析了各种秸秆类生物质的光合生物产氢能力及其与产氢菌种生长之间的相关关系,得到了秸秆类生物质酶解及光合生物产氢的相关动力学方程。实验结果表明,4种秸秆类生物质的酶解效果与产氢能力从大到小依次为小麦秸秆、玉米秸秆、高粱秸秆、棉花秸秆,酶解后还原糖质量浓度分别达到了19.88、15.72、14.04、9.41 g/L,累积产氢量分别达到了515.7、362、194.8、123.95 m L,且在菌种生长的对数期产氢速率达到最大。同时,利用类分形动力学揭示了秸秆类生物质酶解动力学参数与还原糖质量浓度及累积产氢量成正比例关系,为进一步完善秸秆类生物质光合生物产氢工艺理论和技术提供了参考。

Dynamics of Biomass Straw Enzymolysis and Photosynthetic Characteristics of Biological Hydrogen Production

An cellulase hydrolysis process and an anaerobic fermentation process to produce hydrogen from four kinds of common straw biomass were investigated. The hydrolysis of four kinds of common straw biomass and capacity of their hydrogen production were experimented by using fractal-like kinetic. And the correlation between the ability of biological hydrogen production and the growth of photosynthetic bacteria was analyzed. As a result, the kinetic equations of straw biomass hydrolysis and hydrogen production were got. The experimental results show that the effect of straw biomass hydrolysis and the capacity of hydrogen production from large to small are wheat straw, corn straw, sorghum straw and cotton stalk. With the enzymatic degradation, the reduction sugar content are 19.88g/L, 15.72g/L,14.04g/L and 9.41g/L. The cumulative amounts of hydrogen production are 515.7mL, 362mL, 194.8mL and 123.95mL. In this period, the hydrogen production rate reaches maximum in exponential phase of photosynthetic bacteria. Meanwhile, the fractal-like kinetic brings out the direct proportion among the straw biomass hydrolysis kinetic parameter, the reduction sugar content and the cumulative amount of hydrogen production. It lays a reference for further improving the process theory and technology of hydrogen production with straw biomass.