| 淡水小龙虾头中甲壳素的EDTA法提取工艺研究 |
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| 引用本文： | 窦 勇,胡佩红,王 芳.淡水小龙虾头中甲壳素的EDTA法提取工艺研究[J].农业科技与装备,2014(3):59-61,63. |
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| 作者姓名： | 窦 勇 胡佩红 王 芳 |
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| 作者单位： | [1]江苏财经职业技术学院,江苏淮安223003 [2]淮安正昌饲料有限公司,江苏淮安223005 |
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| 基金项目： | 江苏财经职业技术学院大学生实践创新项目（综合利用淡水小龙虾加工废弃物开发新产品）;江苏省淮安市科技局科技支撑计划农业项目（淡水小龙虾加工废弃物综合利用技术研究与新产品开发.SN1174） |
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| 摘 要： | 以淡水小龙虾头为原料,采用EDTA法提取甲壳素,在单因素试验的基础上,通过正交试验筛选最佳工艺条件。试验结果表明:以13%的EDTA溶液作提取溶剂,在反应温度30℃、pH 12、投料比1∶15、反应25 min的条件下,提取效果最佳,提取率达24.05%,反应前后的脱钙和脱蛋白率分别为100.0%和98.6%。
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| 关 键 词： | 甲壳素 小龙虾头壳 提取率 |
Study on the Extraction of Chitin from Procambarus Clarkii Head Shell with EDTA Method |
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| DOU Yong,HU Peihong,WANG Fang.Study on the Extraction of Chitin from Procambarus Clarkii Head Shell with EDTA Method[J].Agricultural Science & Technology and Equipment,2014(3):59-61,63. |
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| Authors: | DOU Yong HU Peihong WANG Fang |
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| Institution: | DOU Yong, HU Peihong, WANG Fang |
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| Abstract: | Taking Procambarus clarkii head shell as raw material, the chitin is extracted using EDTA method. On the basis of single factor test, the best conditions were deselected by orthogonal test. The results show that when the reaction temperature is 30 ℃, pH 12, the use of 13% EDTA solution as extraction solvent feed ratio of 1：15, reaction time 25min, the best extraction, the extraction rate is 24.05%, before and after reaction decalcification and removal of protein rates is 100%and 98.6%, respectively. |
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| Keywords: | EDTA Chitin EDTA Procambarus clarkii head shell Extraction rate |
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