| 鱼腥草愈伤组织的诱导技术 |
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| 引用本文： | 傅华英.鱼腥草愈伤组织的诱导技术[J].甘蔗(福建),2010(4):225-227. |
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| 作者姓名： | 傅华英 |
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| 作者单位： | 福建农林大学农业部甘蔗遗传改良重点开放实验室,福建福州350002 |
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| 摘 要： | 以鱼腥草茎秆和叶片为外植体,进行外植体消毒和愈伤组织诱导条件的研究。结果表明：外植体在0.1%升汞中浸泡10 min可达到良好的消毒效果,污染率低于2%;愈伤诱导所用的茎秆和叶片两种器官中,适宜的外植体是茎秆,其诱导率可达31.25%;愈伤诱导时,24 h暗培养比12 h黑暗与12 h光照交替培养诱导率高;在含2,4-D或6-BA的3种培养基中,仅在MS＋2.0 mg.L-16-BA＋6.0 g.L-1琼脂＋30 g.L-1蔗糖的诱导培养基中诱导出了愈伤组织,诱导率最高为28.57%。
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| 关 键 词： | 鱼腥草 愈伤组织 诱导 |
Callus induction technology of Houttuynia cordata Thunb. |
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| FU Hua-ying.Callus induction technology of Houttuynia cordata Thunb.[J].Sugarcane,2010(4):225-227. |
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| Authors: | FU Hua-ying |
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| Institution: | FU Hua-ying(Key Lab of Sugarcane Genetic Improvement,Ministry of Agriculture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | Taking the stem and leaf of Houttuynia cordata Thunb.as explants,the explants sterilization and the callus induction conditions were researched.Results were as follows： explants were putting in 0.1% HgCl2 for 10 minutes,the rate of pollution is inferior to 2%;comparing callus induction of stem and leaf suitable explant is stem,the rate of callus induction is 31.25%,Callus induction training environment 24 h dark is better than 12 h dark and 12 h light,In three kinds of 2,4-D or 6-BA mediam,only in MS＋2.0 mg·L-1 6-BA＋6.0 g·L-1 agar＋30 g·L-1 sucrose of induction medium the callus induction rate is highest,reaching 28.57%. |
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| Keywords: | Houttuynia cordata Thunb callus induction |
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