Molecular characterization and comparative analysis of four new genes from Sec2 locus encoding 75K γ-secalins of rye species |
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Authors: | Qi-Jiao Chen Zhong-Wei Yuan Lian-Quan Zhang Ze-Hong Yan Zhi-Guo Xiang Yong-Fang Wan You-Liang Zheng Deng-Cai Liu |
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Institution: | aTriticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China;bKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, Sichuan 625014, China;cRothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK |
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Abstract: | Using a PCR-based strategy, four new 75K γ-secalin genes were isolated from Secale cereale, Secale vavilovii, Secale sylvestre, and Secale strictum in genus Secale (rye). Based on amino acid sequences, the primary structure of the 75K γ-secalin subunits was demonstrated, which was composed of four main structural regions: (a) a conservative 19 amino acids signal peptide, (b) a steady short N-terminal region of 12 amino acids containing a cysteine residue, (c) a repetitive domain, which began with the conservative tetrapeptides PQ3 and was rich in glutamine and proline. PFPQ1−2(PQQ)1−2 was the core repeat motif in the repetitive region. Besides amino acid substitutions, this region showed variations in length due to the insertion and deletion events. In the repetitive region of EF432549 (Secale strictum), there were two octapeptides (PFPQQPQQ and PVPQQSQQ) insertions. On the contrary, deletion events of two residues (QT) took place in EF432546 (Secale sylvestre). Accounting for the amino acid replacement, an extra cysteine residue appeared in the repetitive region of EF432546, which did not exist in other genes, and (d) a conserved 143 amino acids C-terminal domain including eight cysteine residues. The implications of the results for quality improvement are discussed. |
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Keywords: | Rye secalins Secale Sec2 gene Storage proteins |
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