Optimization of germination conditions to enhance hydroxyl radical inhibition by water-soluble protein from stress millet |
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| Institution: | 1. The R&D Centre of Yurun Food Group Co. Ltd., Jiangsu, Nanjing 210041, People''s Republic of China;2. The Key Laboratory of Food Science and Safety, Ministry of Education and School of Food Science and Technology, Jiangnan University, No. 1800, Lihu Road, Jiang Su, Wuxi 214122, People''s Republic of China;3. School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, People''s Republic of China;4. College of Information Engineering, Shanghai Maritime University, Shanghai 200135, People''s Republic of China;5. College of Quarter-master Technology, Jilin University, Changchun 130062, People''s Republic of China;1. Department of Oral Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan;2. Department of Medical Engineering, Faculty of Engineering, Tokyo City University, Tokyo 158-8557, Japan;1. Department of Food Science and Institute for Food, Nutrition and Well-being, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa;2. Materials Research Department, iThemba LABS, NRF, Somerset West 7129, South Africa;3. Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa;1. College of Life Science, Shanxi University, Taiyuan 030006, China;2. Department of Food Science, Cornell University, Ithaca, NY 14850-7201, USA;1. Department of Entomology and Neuroscience Program, Michigan State University, East Lansing, MI 48824, United States;2. Department of Plant Molecular Biology & Ecology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel;3. Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, United States |
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| Abstract: | This study aimed to further increase the yield of the hydroxyl radical inhibitory water-soluble protein from stress germinated millet. For this, the effects of the sprouting conditions (temperature, time and pH of stress medium) on the hydroxyl radical inhibition were investigated carefully by single-factor experiments and statistical experimental designs, which included a fractional factorial design, steepest ascent experiments, a central composite experimental design and response surface methodology. The optimal conditions were identified as temperature 28 °C, culture time 54 h and stress medium pH 7.5. Under the optimum conditions, the highest inhibition (60.38%) was achieved. |
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