Physicochemical Studies on Resistant StarchIn VitroandIn Vivo |
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Authors: | P Cairns VJ Morris RL Botham SG Ring |
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Institution: | Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, U.K. |
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Abstract: | Resistant starch (RS), producedin vitroby hydrolysis of retrograded pea starch gels and amylose gels by porcine pancreaticalpha-amylase, was characterised by X-ray diffraction, size exclusion chromatography and methylation analysis. These techniques showed that RSin vitroconsisted of semi-crystalline, mostly linear material that was present in two main molecular size subfractions (DPn>100 andDPn20–30) with a third, minor subfraction (DPn≤5). The extent of retrogradation of amylose was found to be of primary importance in determining the RS content of starch. Analysis ofin vivoRS, recovered during an ileostomy study, produced results that were similar to those obtained from RSin vitro. Anin vitromodel for the structure of resistant starch is proposed. |
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Keywords: | resistant starch amylose alpha-amylase ileostomy |
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