| 水稻茉莉素受体基因OsCOI1a的克隆与功能研究 |
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| 引用本文： | 欧阳诗柳,任春梅.水稻茉莉素受体基因OsCOI1a的克隆与功能研究[J].作物研究,2014(4):337-340. |
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| 作者姓名： | 欧阳诗柳 任春梅 |
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| 作者单位： | 湖南农业大学生物科学技术学院,长沙410128 |
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| 基金项目： | 国家自然科学基金项目（30770195）. |
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| 摘 要： | 将拟南芥茉莉素受体基因COI1在水稻中的同源基因OsCOI1a构建到植物双元表达载体pMYC2,导入拟南芥茉莉素受体突变体coi1-1中,采用Western blot检测OsCOI1a基因的蛋白表达水平,通过观察突变体花粉的活性和果荚的育性,检测OsCOI1a对coi1-1突变体的互补情况,研究OsCOI1a基因的功能。结果表明,OsCOI1a基因能够在拟南芥coi1-1突变体中正确表达,并能部分互补雄性不育的表型,表明OsCOI1a基因在水稻茉莉素信号通路中可能起茉莉素受体作用。
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| 关 键 词： | 水稻 茉莉素 OsCOI1a基因 克隆 |
Cloning and Function of the Rice Jas monate Receptor Gene OsCOI1a |
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| OUYANG Shi-liu,REN Chun-mei.Cloning and Function of the Rice Jas monate Receptor Gene OsCOI1a[J].Crop Research,2014(4):337-340. |
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| Authors: | OUYANG Shi-liu REN Chun-mei |
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| Institution: | ( College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China) |
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| Abstract: | We cloned OsCOI1a into the expression vector pMYC2,and transformed it into Arabidopsiscoi1-1 mutants. We tested the protein expression levels by western blot,observed the activity of pollen and the sterility of siliques in coi1-1 mutants transformed with OsCOI1 a,and found out that OsCOI1 a could partly complement the phenotype of the sterility of Arabidopsis coi1-1 mutants. All these results suggested that OsCOI1 a might be a receptor of jasmonates in rice. |
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| Keywords: | Rice Jasmonate receptor Gene OsCOI1 a Cloning |
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