抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选

条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11 KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份笹锦/Habataki//笹锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S(感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。

Development of Functional Marker for Rice stripe virus Resistant Gene STV11 and Resistant Germplasm Selection in japonica Rice

The Rice stripe virus (RSV) causes one of the most serious viral diseases of rice (Oryza sativa L.). STV11 is a rice stripe virus resistant gene derived from india rice Kasalath. We developed a simple PCR based functional marker named qstv11 which targets the functional six bp delection polymorphism at STV11KAS. The genotype on STV11 and rice stripe virus resistance of 299 varieties was analyzed, including five resistant varieties, five susceptible varieties, 41 varieties from Liaoning Province, 17 Japanese japonica varieties, 142 recombinant inbred lines (Akihikari/Qishanzhan,population A), 85 backcross recombinant inbred lines (Sasanishiki/Habataki//Sasanishiki population,B) and 4 parents. The newly developed functional marker was highly efficient in discriminating resistant genotypes from susceptible ones and showed perfect co segregation with the trait of rice stripe virus resistance in the test varieties. One variety “Kangyan 100” was identified with STV11 R from Liaoning varieties. All the varieties from Japanese japonica varieties were susceptible to rice stripe virus. Twenty four and six varieties were identified with STV R from population A and population B, respectively. Several varieties with STV11 R type with japonica background were detected.