利用回交重组自交系定位稻米赖氨酸含量的基因座位

 利用日本晴（粳稻）/Kasalath（籼稻）//日本晴组合衍生的98个回交重组自交系（BILs）株系和具有245个RFLP标记的遗传图谱，在浙江和海南2个环境条件下，开展了精米赖氨酸含量的QTL定位。精米赖氨酸含量在浙江和海南均表现连续变异和超亲分离。在第6染色体发现2个具有显著遗传主效应的QTL(qLYS6 1和qLYS6 2），表型贡献率分别为2708%和4756%。qLYS6 1 还具有显著的环境互作效应。qLYS6 1的增效基因来自Kasalath，而qLYS6 2的增效基因来自日本晴。未检测到显著的上位性效应。

QTLs Mapping for Lysine Content of Rice Using Backcross Inbred Lines

A backcross inbred lines (BILs) population consisting of 98 lines derived from a cross of Nipponbare (japonica)/Kasalath (indica) Nipponbare was used to detect QTLs controlling the lysine content of milled rice in two distinct environments, Zhejiang and Hainan Provinces, China. The lysine content of BILs ranged from 2.19 mg/g to 4.03 mg/g in Zhejiang and 2.39 mg/g to 4.29 mg/g in Hainan, with continuous variation and transgressive segregation. Two putative QTLs (qLYS6-1 and qLYS6-2) with genetic main effects were detected on chromosome 6, which contributed to 27.08 % and 47.56% of the total phenotypic variations, respectively. qLYS6-1 was also detected to have significant genotype X environment interaction effects. The additive effect of qLYS6-1 came from Kasalath, while qLYS6-2 from Nipponbare. In addition, no additive additive epistasis for QTLs was detected.