抗除草剂基因导入培矮64S实现杂交水稻制种机械化的初步研究

 利用农杆菌介导的高效遗传转化技术，成功地将Bar基因转入两系杂交稻的不育系培矮64S。转基因植株具有明显的Basta抗性，分子检测进一步证明Bar基因已整合到水稻染色体上。模拟制种的成功，表明机械化制备杂交水稻种子是可行的。讨论了实现机械化制备杂交水稻种子所需解决的各种问题。

Primary Study on Mechanization of Seed Production of Hybrid Rice by Inducing Bar Gene to Pei′ai 64S

Using a high efficient rice transformation system mediated byAgrobacterium tumefaciens, Bar gene was introduced into a photoperiod-temperature sensitive genetic male sterile line Pei′ai 64S. The transgenic plants showed the resistance to herbicide Basta. PCR and Southern blot analysis indicate that Bar gene was inserted in genomic DNA of Pei′ai 64S. Simulation on seed production of hybrid rice using transgenic Pei′ai 64S as a female parent was also in successful. This result proved the feasibility of mechanization to produce hybrid rice seeds via gene transformation. The problems on practical utilization of this protocol are discussed.