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| 汕优63杂合性的RAPD和AFLP分析 | |
| 引用本文： | 何光华,唐梅,裴炎,杨光伟,郑家奎,候磊.汕优63杂合性的RAPD和AFLP分析[J].中国水稻科学,2000,14(3):177-178. |
| 作者姓名： | 何光华 唐梅 裴炎 杨光伟 郑家奎 候磊 |
| 作者单位： | 1. 西南农业大学,生物技术中心,重庆,北碚,400716;四川省农业科学院,水稻高粱研究所,四川,泸州,646100 2. 西南农业大学,生物技术中心,重庆,北碚,400716 3. 四川省农业科学院,水稻高粱研究所,四川,泸州,646100 |
| 基金项目： | 四川省生物技术重点项目，四川省应用基础研究资助 项目 |
| 摘 要： | 利用57个RAPD引物和15个AFLP引物分析了明恢63与珍汕97A之间1209个位点的杂合性。结果表明，57个RAPD引物能扩增出394条带，平均每个引物扩增6.9条，多态性带129条，汕优63的杂合性为32.74%；15个AFLP引物共扩增出815个位点，平均每个引物扩增54.33条，多态性带289条，汕优63的杂合性为35.46%。1209个位点显示汕优63的杂合性为34.57%。 |
| 关 键 词： | 杂交水稻 随机扩增多态性DNA 扩增片段长度多态性 杂合性 |
| 收稿时间： | 1900-01-01; |
Analysis of Heterozygosity in Shanyou 63 Based on RAPD and AFLP |
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| HE Guang-hua,TANG Mei,PEI Yan,YANG Guang-wei,ZHENG Jia-kui,HOU Lei.Analysis of Heterozygosity in Shanyou 63 Based on RAPD and AFLP[J].Chinese Journal of Rice Science,2000,14(3):177-178. | |
| Authors: | HE Guang-hua TANG Mei PEI Yan YANG Guang-wei ZHENG Jia-kui HOU Lei |
| Institution: | (Center of Biotechnology; Southwest Agricultural University; Chongqing 400716; China; Rice and Sorghum Research Institute; Sichuan Academy of Agricultural Sciences, Luzhou 646100, China) |
| Abstract: | The heterozygosity of 1209 molecular markers was surveyed between Minghui 63 and Zhenshan 97A for RAPD analysis using 57 primers and AFLP analysis using 15 primers. The results showed that, 57 RAPD primers amplified 394 bands with a mean of 6.9 bands per primer, resulting in 129 bands of polymorphisms heterozygosity of Shanyou 63 is 32.74 percent, 15 AFLP primers amplified 815 loci with a mean of 54.33 loci per primer, resulting in 289 loci of polymorphisms, the heterozygosity is 35.46 percent. Therefore 1209 markers indicated the heterozygosity of Shanyou 63 is 34.57 percent. |
| Keywords: | hybrid rice random amplified polymorphic DNA amplified fragment length polymorphism heterozygosity |
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