T-DNA插入水稻突变体库的叶绿素和净光合速率变化

 从T-DNA插入的水稻（Oryza sativa L. subsp. japonica)中花11突变体库中挑选21个叶色表型发生明显变异的株系，在孕穗期测定了其倒2叶的叶绿素含量及其光合速率。结果表明突变体间叶绿素含量差别较大，有两个突变体的叶绿素b含量非常低，其他突变体叶绿素a、叶绿素b和叶绿素(a+b)含量间呈线性相关。而且，叶绿素含量与叶色有关，多数表现出色浅的叶绿素含量低，色深则叶绿素含量高；突变体的净光合速率普遍较对照低，并且叶绿素含量与净光合速率没有相关性，但叶色浓的突变体，净光合速率相对高些，叶色偏淡的材料，其净光合速率的变化极大。矮秆突变体的净光合速率比较低。最后讨论了这些突变体在生理学和功能基因组学研究中的作用。

Chlorophyll Contents and Net Photosynthetic Rates of T-DNA Inserted Rice Mutant Population

Twenty-one lines with phenotype variation were scr eened from T-DNA inserted population of rice (Oryza sativa L. subsp. japo nica cv. Zhonghua 11), chlorophyll content and net photosynthetic rate were m easured at booting stage. There were obvious differences in chlorophyll content among lines, two lines with low content of chlorophyll b were found. Linear rela tionships existed between the contents of chlorophyll a, chlorophyll b and ch lorophyll (a+b) respectively. Leaf color was related to the content of chlorop hyll. Usually, lines with dark green leaves had higher chlorophyll contents and those with light green leaves had lower contents, while the relation to net phot osynthetic rate was complicated. The net photosynthetic rate of all lines was lo wer than that of the control. It was also found that the net photosynthetic rate of several dwarf lines was very low.The role of these mutants on plant physiolo gy and rice functional genomics research were discussed.