不同温光条件下水稻抽穗期QTL的定位与分析

以广陆矮4号为受体,日本晴为供体的85个染色体单片段代换系群体为试验材料,通过单因素方差分析和Dunnett多重比较,测验单片段代换系与广陆矮4号之间抽穗期的差异,对代换片段上抽穗期相关的QTL进行了定位。以P≤0.001为阈值,在南京和海南不同温光条件下共定位到40个抽穗期相关的QTL。其中,21个QTL在2个环境中均被检测到;15个QTL只在南京环境中被检测到;4个QTL只在海南环境中被检测到。南京环境中定位到的36个抽穗期相关QTL,其加性效应值变化范围为2.8d~15.7d,加性效应百分率变化范围为3.8%~21.1%;海南环境中定位到的25个抽穗期相关QTL,加性效应值变化范围为1.8d~12.1d,加性效应百分率变化范围为1.7%~11.3%。这些QTL的定位,为进一步精细定位并克隆相应主效QTL和优异品种特定环境下的生育期改良奠定了基础。

QTL Analysis for Heading Date in Rice (Oryza sativa L.) Under Different Temperatures and Light Intensities

QTLs for heading date were identified with 85 rice chromosome single segment substitution lines (CSSSLs) derived from Nipponbare as donor parent in the background of Guanglu’ai 4, for means comparisons between CSSSLs and the recipient parent Guanglu’ai 4 by one way analysis of variance and Dunnett’s test. A total of 40 QTLs for heading date were identified on all 12 chromosomes under two different temperatures and light intensities with a significance of P≤0.001. Twenty one QTLs for heading date were mapped in the two environments, 15 QTLs only in Nanjing, and four only in Sanya. Thirty six QTLs were mapped in Nanjing, the additive effects of which ranged from 28 d to 15.7 d, the additive effect percentages from 3.8% to 21.1%; 25 QTLs were mapped in Sanya, the additive effect of which ranged from 1.8 to 12.1 d, the additive effect percentages from 1.7% to 11.3%. The results are important for the QTLs cloning and breeding of new high quality rice variety in different environments.