提高粳稻体细胞培养绿苗频率的研究

本试验结果证明:(1)通过异地、异季种植和温度预处理等可以使那些难以分化的粳稻材料有效地提高愈伤组织诱导率和绿苗分化率。(2)在 N_6培养基中,降低生长素的浓度,添加水解乳蛋白和水解核酸也可提高愈伤组织的绿苗分化率,但其效果远不及前者。

A Study On Increasing Frequencies of Somatic Tissue-Derived Plants in Rice

(1)Oryza sativa(sinica type cv.) materials, which are difficult for differentiation in this region, effectively increased their frequencies of callus induction and plantlet differentiation through the pretreatment of different temperatures or growth in other regions of China. (2)Lower auxin level in basal culture medium N6,with additional hydrolytic lactalbamin and degradated nucleic acid,also increased the frequencies of callus formation and plantlet differentiation,but not so effectively as (1).