东北粳稻分蘖期耐冷性鉴定及SSR标记关联分析

 以140份东北粳稻品种（系）为材料进行分蘖期耐冷鉴定，用84个SSR标记对耐冷性差异较大的96份材料进行基于SSR标记的关联分析。结果表明，供试材料强耐冷品种（系）占20%，中间型（系）占30.71%，不耐冷品种（系）占49.28%；分蘖期耐冷性与结实率和单株穗重呈极显著正相关，与生育期呈极显著负相关；关联分析得到18个位点与分蘖期耐冷性关联，其中标记RM214，RM445，RM506，RM106，RM215，RM182，RM270和RM229与前人研究获得的水稻耐冷性QTL定位结果吻合。这些标记可作为东北粳稻耐冷性资源筛选重点选择的标记。 还通过优异等位基因的挖掘，得到一系列的优异等位变异及载体材料。

Association Analysis of Rice Cold Tolerance at Tillering Stage with SSR Markers in japonica Cultivars in Northeast China

The cold tolerance of rice at the tillering stage was evaluated with 140 rice cultivars(lines) from Northeast China as materials and association analysis was conducted among 96 selected materials which differed in cold tolerance with 84 SSR markers. The experimental results showed: there were 20.0% of rice materials with better cold tolerance, 30.71% with intermediate cold tolerance and 49.28% with worse cold tolerance; Correlation analysis showed that rice cold tolerance at the tillering stage was significantly positively correlated with seed setting rate and single panicle weight. Rice cold tolerance at the tillering stage and growth duration were significantly negatively correlated; Eighteen markers associated with rice cold tolerance were detected through the association analysis, markers like RM214，RM445，RM506，RM106，RM215，RM182，RM270 and RM229 were mutual authentication with family based linkage mapping. These markers can be used for selection of rice cold tolerance resources in Northeast China. Finally, a serial of elite alleles, loci and their carrier materials were screened out by anglicizing these associated markers.