琼脂糖凝胶电泳在水稻EcoTILLING技术中的应用

 为构建低成本的基于琼脂糖凝胶电泳的EcoTILLING技术体系，用于水稻及其他生物自然群体变异的检测，对基于琼脂糖凝胶的EcoTILLING技术进行了优化。结果表明，从芹菜中提取的CEL Ⅰ核酸酶粗提物结合琼脂糖凝胶电泳检测技术，可以成功地检测到目的基因位点的突变。利用该技术，在云南地方水稻品种大吊糯中检测到2 个SNPs。

Application of Agarose Gel Electrophoresis in Rice EcoTILLING

To establish a cheaper EcoTILLING system based on agarose-gel-electrophoresis for the detection of natural variation of rice or other plant species,a convenient EcoTILLING technique combined agarose gel electrophoresis with active rude CELⅠ nuclease extract from celery was constructed. By using the EcoTILLING technique,two SNPs were detected in a Yunnan conventional rice variety Dadiaonuo.