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| 一个水稻类病条纹斑突变体的鉴定和遗传定位 | |
| 引用本文： | 马健阳,陈孙禄,张建辉,董彦君,滕胜.一个水稻类病条纹斑突变体的鉴定和遗传定位[J].中国水稻科学,2011,25(2):150-156. |
| 作者姓名： | 马健阳 陈孙禄 张建辉 董彦君 滕胜 |
| 作者单位： | 1. 上海师范大学,生命与环境科学学院,植物遗传与功能基因研究室,上海,200234;中国科学院,上海生命科学研究院,植物生理生态研究所,上海,200032 2. 中国科学院,上海生命科学研究院,植物生理生态研究所,上海,200032;南京农业大学,作物遗传与种质创新国家重点实验室,江苏,南京,210095 3. 上海师范大学,生命与环境科学学院,植物遗传与功能基因研究室,上海,200234 4. 中国科学院,上海生命科学研究院,植物生理生态研究所,上海,200032 |
| 基金项目： | 国家自然科学基金资助项目(30970246,30770182,30971552) |
| 摘 要： | 粳稻品种嘉花1号经60Co γ射线辐射诱变后获得一个水稻类病斑突变体MR07, 暂命名为lms1。该突变体的病斑在全生育期均表现，属于扩散型类病斑突变体。生理和组织化学分析表明，该突变体在高温条件下（30℃）培养时只表现为白色条斑，低温条件下（20℃）培养时表现出细胞自主性死亡的坏死病斑。遗传分析表明，该突变体受1对隐性核基因控制。以lms1突变体与籼稻9311、培矮64S杂交的两个F2分离群体作为定位群体，利用SSR标记和Indel标记将该基因定位在第6染色体上，位于标记InDel1和MM0112-4之间，其物理距离为400 kb。 |
| 关 键 词： | 水稻 类病斑突变体 基因定位 |
| 收稿时间： | 2010-08-18; |
Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice |
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| MA Jian-yang,CHEN Sun-lu,ZHANG Jian-hui,DONG Yan-jun,TENG Sheng.Identification and Genetic Mapping of a Lesion Mimic Stripe Mutant in Rice[J].Chinese Journal of Rice Science,2011,25(2):150-156. | |
| Authors: | MA Jian-yang CHEN Sun-lu ZHANG Jian-hui DONG Yan-jun TENG Sheng |
| Institution: | MA Jian-yang1,2,CHEN Sun-lu2,3,ZHANG Jian-hui1,DONG Yan-jun 1,TENG Sheng2,(1Laboratory of Plant Genetics and Functional Gene,College of Life and Environment Science,Shanghai Normal University,Shanghai 200234,China,2Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,3National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,) |
| Abstract: | A lesion mimic stripe mutant was obtained from the M2 progeny of a japonica variety Jiahua 1 by 60Co γ radiation,temporarily designated as lms1 (lesion mimic stripe 1). The mutant displayed lesion mimic in its whole life, and belonged to propagation type. Physiological and histochemistry analysis suggested that the mutant displayed a phenotype of white stripe at a high temperature (30℃) and lesion mimic caused by cell initiative death at a low temperature(20℃)，respectively. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene. In addition, using SSR markers and an F2 segregating population derived from two crosses(lms1/9311 and Pei’ai 64S/lms1), the lms1 gene was mapped between Indel1 and MM0112-4 with a physical distance of 400 kb on chromosome 6 in rice. |
| Keywords: | rice lesion mimic mutant gene mapping |
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