| 分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性 |
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| 引用本文： | 兰艳荣,王俊义,王弋,牟同敏.分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性[J].中国水稻科学,2011,25(2):169-174. |
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| 作者姓名： | 兰艳荣 王俊义 王弋 牟同敏 |
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| 作者单位： | 华中农业大学,作物遗传改良国家重点实验室/国家植物基因研究中心/国家作物分子育种中心,湖北,武汉,430070 |
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| 基金项目： | 国家863计划资助项目(2010AA101304) |
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| 摘 要： | 以华201S为受体亲本，以含Xa21和Xa7基因的抗白叶枯病材料华恢20为供体亲本，进行1次杂交、3次回交和3次以上自交，每个世代用基因内标记PTA248对Xa21进行分子检测，用连锁标记STSP3（与Xa7的遗传距离为0.9 cM）对Xa7进行检测。新选育的4个株系YR7009(Xa21)、YR7014(Xa21)、YR7016(Xa7)和YR7023(Xa7/Xa21)在生育期、主要农艺性状、育性转换特性方面都与华201S基本相似。YR7009(Xa21)和YR7014(Xa21)对PXO61、PXO99、ZHE173和GD1358等4个菌株具有良好的抗性，而对FuJ和YN24不具有抗性。YR7016(Xa7)和YR7023(Xa7/Xa21)对6个菌株都表现良好的抗性，尤其是聚合双基因的YR7023(Xa7/Xa21)对6个菌株都表现高抗。表明已经将广谱白叶枯病抗性基因Xa21和Xa7渗入到华201S中，成功地改良了华201S的白叶枯病抗性。
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| 关 键 词： | 水稻 光温敏核不育系 分子标记辅助选择 白叶枯病 抗性改良 |
| 收稿时间： | 2010-07-17; |
Improvement of Rice Bacterial Blight Resistance of Hua 201S,an Elite Photo-Thermo-Sensitive Genic Male Sterile Line,by Molecular Marker-Assisted Selection |
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| LAN Yan-rong,WANG Jun-yi,WANG Yi,Mou Tong-min.Improvement of Rice Bacterial Blight Resistance of Hua 201S,an Elite Photo-Thermo-Sensitive Genic Male Sterile Line,by Molecular Marker-Assisted Selection[J].Chinese Journal of Rice Science,2011,25(2):169-174. |
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| Authors: | LAN Yan-rong WANG Jun-yi WANG Yi Mou Tong-min |
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| Institution: | LAN Yan-rong,WANG Jun-yi,WANG Yi,MOU Tong-min (National Key Laboratory of Crop Genetic Improvement/National Center of Plant Gene Research/National Center of Crop Molecular Breeding,Huazhong Agricultural University,Wuhan 430070,China,) |
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| Abstract: | Hua 201S is an elite photo-thermo-sensitive genic male sterile(PTGMS) line,which was used for developing two-line hybrid rice.A bacterial blight resistant breeding line 'Huahui 20' containing Xa21 and Xa7 genes as donor parent was crossed with Hua 201S.F1 was backcrossed to Hua 201S for three times.The BC3F1 progenies were advanced to BC3F3 by selfing.The primers PTA248 for Xa21 and STSP3(genetic distance to Xa7 is 0.9 cM) for Xa7 were used for molecular marker-assisted selection in each progeny.The fertili... |
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| Keywords: | rice photo-thermo-sensitive genic male sterile line molecular marker-assisted selection bacterial blight resistance improvement breeding |
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