旱稻品种IRAT109辐照诱变突变体的筛选和鉴定

利用γ射线辐照处理旱稻品种IRAT109的干种子样品,加代至M2后混收种子,分批在水田和干旱胁迫条件下种植M3群体,通过苗期、分蘖期和抽穗期的观察获得突变单株,加代繁育至M5后考查可稳定遗传的突变性状,共获得260份不同性状的突变体。介绍了其中部分突变体在叶片、茎秆、穗部、籽粒形态和抗旱性等方面表现,配制个别突变体与野生型的F2群体并初步观察分离比例。讨论了筛选抗旱性相关突变体的技术困难和该套突变体群体的应用价值。

Development and Identification of Mutants Induced by γ-ray Irradiation from an Upland Rice Variety IRAT109

Dry seed samples of the upland rice variety IRAT109 were treated with γ ray irradiation. Mixed seed bulk was collected from M2 plants. Mutated individuals were collected at seedling, tillering and heading stages from M3 population under dry cultivation and traditional cultivation for several seasons. The mutant population had 260 lines with stably inherited mutations identified in M5 generation. A proportion of the mutants were described including mutants in leaves, culms, panicles, grains, and drought tolerance related traits. The segregation ratios of F2 population were observed for a couple of mutants. In addtion, the problems on screening mutations for drought tolerance and the utility of this mutant population were discussed.