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| 水稻稻瘟病菌胁迫应答cDNA片段的表达及定位 | |
| 引用本文： | 饶志明,董海涛,庄杰云,柴荣耀,樊叶杨,李德葆,郑康乐.水稻稻瘟病菌胁迫应答cDNA片段的表达及定位[J].中国水稻科学,2003,17(2):105-108. |
| 作者姓名： | 饶志明 董海涛 庄杰云 柴荣耀 樊叶杨 李德葆 郑康乐 |
| 作者单位： | 1. 中国水稻研究所,国家水稻改良中心,浙江,杭州,310006 2. 浙江大学,农业部植物病理学重点实验室,浙江,杭州,310029 3. 浙江省农业科学院,植物保护研究所,浙江,杭州,310021 |
| 基金项目： | 浙江省自然科学基金资助项目 ( 3 0 0 0 0 6)，国家自然科学基金资助项目 ( 3 0 0 70 40 0 )，农业部水稻生物学重点开放实验室资助项目 ( 0 0 0 2 0 3 ) |
| 摘 要： | 以抗稻瘟病品系G205为材料，应用cDNA微阵列分别获得了一个受稻瘟病菌诱导的含NBS LRR的cDNA克隆（暂命名为RIM1, rice induced by Magnaporthe grisea）和一个受稻瘟病菌抑制的编码腈水解酶（Nitrilase）的cDNA克隆（暂命名为NIT），并通过Northern得到证实。RFLP分析将RIM1和NIT分别定位于水稻第2和第3染色体上，它们均位于控制水稻稻瘟病部分抗性QTL区间。 |
| 关 键 词： | 水稻 cDNA微阵列 腈水解酶 基因定位 |
| 文章编号： | 1001-7216(2003)02-0105-04 |
| 收稿时间： | 1900-01-01; |
Expression and Mapping of cDNA Fragments Responsive to Magnaporthe grisea in Rice |
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| RAO Zhi ming ,DONG Hai tao ,ZHUANG Jie yun ,CHAI Rong yao ,FAN Ye yang ,LI De bao ,ZHENG Kang le.Expression and Mapping of cDNA Fragments Responsive to Magnaporthe grisea in Rice[J].Chinese Journal of Rice Science,2003,17(2):105-108. | |
| Authors: | RAO Zhi ming DONG Hai tao ZHUANG Jie yun CHAI Rong yao FAN Ye yang LI De bao ZHENG Kang le |
| Institution: | RAO Zhi ming 1,DONG Hai tao 2,ZHUANG Jie yun 1,CHAI Rong yao 3,FAN Ye yang 1,LI De bao 2,*,ZHENG Kang le 1,* |
| Abstract: | The expression profiles of a resistant line G205 derived from a RIL population of Zhong 156×Gumei 2 in response to Magnaporthe grisea were investigated by using cDNA microarray containing 2200 expressed sequence tags (ESTs). One cDNA fragment encoding nucleotide binding site (NBS) and leucine rich repeat (LRR) was up regulated and the other cDNA fragment encoding nitrilase associated protein (NIT) was down regulated significantly by the fungus, which were confirmed by Northern blot. They were mapped on chromosomes 2 and 3 by RFLP analysis respectively. Both of them were located on the intervals where QTLs controlling partial resistance to blast had been detected. |
| Keywords: | rice cDNA microarray nitrilase gene mapping Magnaporthe grisea |
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