| 一种EGCG单体纯化新工艺 |
| |
| 引用本文： | 黄磊,杨军国,付杰,夏小欢,裘珺琳,陈旭东.一种EGCG单体纯化新工艺[J].茶叶科学简报,2014(2):12-17. |
| |
| 作者姓名： | 黄磊 杨军国 付杰 夏小欢 裘珺琳 陈旭东 |
| |
| 作者单位： | [1]绍兴市农业科学研究院,浙江绍兴312000 [2]福建省农业科学院茶叶研究所,福建福安355015 |
| |
| 摘 要： | 以粗酯型儿茶素为原料,研究采用大孔吸附树脂和结晶纯化儿茶素单体EGCG的制备工艺。通过比较4种大孔吸附树脂对EGCG的吸附和解吸能力,筛选出最优树脂AB-8,并考察乙醇梯度洗脱等特性,再经过结晶析出EGCG晶体。结果表明:AB-8大孔吸附树脂对EGCG具有较好的吸附选择性,通过10%乙醇洗脱,EGCG产品纯度从54.41%提高到了92.65%,得率达93.38%,结晶后得纯度达98%以上的EGCG单体。
|
| 关 键 词： | EGCG 大孔吸附树脂 结晶 纯化 |
A New Technology for the Purification of EGCG |
| |
| HUANG Lei,YANG Jun-guo,FU Jie,XIA Xiao-huan,QIU Jun-lin,CHEN Xu-dong.A New Technology for the Purification of EGCG[J].Tea Science and Technology,2014(2):12-17. |
| |
| Authors: | HUANG Lei YANG Jun-guo FU Jie XIA Xiao-huan QIU Jun-lin CHEN Xu-dong |
| |
| Institution: | 1.Shaoxing Institute of Agriculture Sciences, Shaoxing, Zhejiang 312003, China ;2.Tea Research lnstitute, Fujian Academy of Agricultural Sciences, Fu＇an, Fujian 355015, China) |
| |
| Abstract: | The way to isolate （-）-epigallocatechin gallate（EGCG）from crude ester catechins with macroporous adsorptive resins in combination with crystallization was investigated in this paper. After comparing the adsorption and desorption capacities among four macroporous adsorptive resins, the optimal resin was chosen, which were further investigated via evaluating the characteristics of gradient elution with ethanol, and finally monomer EGCG was separated by crystallization. The results showed that macroporous adsorptive resins AB-8 possessed the best adsorption selectivity for EGCG. After eluted by 10%ethanol, the purity of EGCG was enhanced from 54.41%to 92.65%, and the yield reached 93.38%. By crystallization, the purity of monomer EGCG was beyond 98%. |
| |
| Keywords: | EGCG macroporous adsorptive resin crystallization purification |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
