| 21份不同木质素含量的苎麻的RAPD聚类分析 |
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| 引用本文： | 李建军,郭清泉,陈建荣.21份不同木质素含量的苎麻的RAPD聚类分析[J].中国麻业,2006,28(3):120-122,127. |
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| 作者姓名： | 李建军 郭清泉 陈建荣 |
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| 作者单位： | [1]湖南农业大学农学院,湖南长沙410128 [2]中国农业科学院麻类研究所,湖南长沙410205 |
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| 基金项目： | 湖南省自然科学基金项目（03JJY3046）.湖南省教育厅重点项目（01A066）.湖南省重大科技专项（03NK1001）.国家“963”资助项目（2001AA241211.2004AA241210）致谢：本文部分工作在湖南农业大学作物基因工程湖南重点实验室完成,在此表示感谢. |
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| 摘 要： | 利用RAPD分析构建了苎麻属植物21份木质素含量不同材料的指纹图谱，从150个随机引物筛选出清晰且多态性高的15个引物，共扩增出85条DNA片段，其中特异性带68条，占80％。同时对退火温度进行了优化，反应条件稳定，重复性好。采用UPGMA法对21个品种扩增的谱带进行遗传聚类分析，在D=0．0760处可分为三类8组，可说明供试品种的亲缘关系。
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| 关 键 词： | 苎麻 木质素含量 RAPD分析 |
| 文章编号： | 1671-3532（2006）03-0120-04 |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006-03-20 |
RAPD Analysis of Lignin Content for 21 Ramie Varieties |
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| LI Jian - jun, GUO Qing -quan, CHEN Jian - rong.RAPD Analysis of Lignin Content for 21 Ramie Varieties[J].Plant Fibers and Products,2006,28(3):120-122,127. |
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| Authors: | LI Jian - jun GUO Qing -quan CHEN Jian - rong |
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| Institution: | 1. Agricultural College of Hunan Agricultural University, Changsha 410128, China; 2. Institute of Bast Fiber Crops, CAAS, Changsha 410205, China |
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| Abstract: | An optional reaction system suitable for the assay and usage of RAPD in ramie was established. The program with fine repoduciblity and high reliability was effectively used for RAPD analysis in ramie genetics and breeding. Genomic DNA of 21 ramie varieties of different lignin content were amplified by RAPD from 150 arbitrary 10 base primers. The results showed that all 85 bands were identi- fied. The polymorphic bands accounted for 80% of the total amplified bands. By drawing ploygenetic relationship through cluster of bands, the experimented accession could be divided among 3 types and 8 groups. |
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| Keywords: | ramie lignin content RAPD analysis |
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