四川小麦品种(系)淀粉颗粒结合蛋白多态性分析

为了解四川小麦品种(系)淀粉颗粒结合蛋白的多样性,对66份四川小麦地方品种、100份新育成品种(系)以及21份小麦骨干亲本材料的淀粉颗粒结合蛋白进行了SDS-PAGE分析。结果表明,在187份供试材料中共分离出7种蛋白条带组合类型,10种不同的蛋白条带,其中5种条带在供试材料中检测到缺失和迁移率改变。114份材料表现为SGP-1A蛋白缺失,而在所有供试的四川小麦地方品种中该蛋白均表达。共4份材料在SGP-1B位点蛋白迁移率改变,18份材料的Wx-1B蛋白条带缺失,8份材料的Wx-1D蛋白条带缺失,仅有1份材料的Wx-1A蛋白条带缺失。通过与中国春缺体-四体(N7AT7B、N7DT7B、N4AT4B)、乌拉尔图小麦(AA)、节节麦(DD)、圆锥小麦(AABB)淀粉颗粒结合蛋白的比较,并结合质谱鉴定,进一步验证了所鉴定条带的准确性。本研究表明,四川小麦新育成品种(系)和骨干亲本比四川小麦地方品种具有更丰富的淀粉颗粒结合蛋白变异类型,鉴定出的变异材料可用于淀粉颗粒结合蛋白功能的研究和小麦淀粉品质育种。

Genetic Diversity of Starch Granule Associated Proteins in Landraces and Cultivars (Lines) of Sichuan

In order to know the genetic diversity of wheat starch granule associated proteins (SGAPs) in Sichuan wheat, 66 wheat landraces, 100 new wheat cultivars (lines) and 21 wheat founder parents from Sichuan were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). A total of seven combinations and ten different protein bands were identified in the 187 materials, and the variations, including unexpressed and the change of mobility, were observed on five bands. The null SGP 1A, the null Wx 1B, and the null Wx 1D were detected in 114, 18, and 8 materials, respectively, but the null Wx 1A was only observed in one material. Based on the comparison with SGAPs in Chinese Spring nullisomic tetrasomic lines (N7AT7B, N7DT7B, N4AT4B), and T. urartu(AA), Ae. tauschii(DD), T. turgidum(AABB), and the results of MALDI TOF MS, we further confirmed that the identification of SGAPs was correct. Our result suggested that the new varieties (lines) showed higher diversity on SGAPs than the landraces of Sichuan, and the materials with the variation of SGAPs could be used in the functional research of SGAPs and the wheat breeding on starch quality.