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陕西小麦品种(系)籽粒黄色素含量基因的检测及其分布
引用本文:张影全,付晓洁,张晓科,张晓卉,张同兴,任万杰,尉 倩.陕西小麦品种(系)籽粒黄色素含量基因的检测及其分布[J].麦类作物学报,2010,30(3):421-426.
作者姓名:张影全  付晓洁  张晓科  张晓卉  张同兴  任万杰  尉 倩
作者单位:1. 西北农林科技大学农学院/国家小麦改良中心杨凌分中心,陕西杨凌,712100
2. 陕西省岐山县农技中心,陕西岐山,722400
基金项目:引进国际先进农业科学技术计划(948计划)项目,西北农林科技大学唐仲英育种基金 
摘    要:为了解陕西小麦Psy-A1和Psy-B1位点控制籽粒黄色素含量基因的等位变异组成和分布,利用其功能标记YP7A、YP7A-2、YP7B-1、YP7B-2和YP7B-3,对176份陕西小麦品种(系)的2个位点等位变异进行检测与分析.结果表明,在Psy-A1位点,陕西小麦品种(系)存在2种等位变异,即Psy-A1a(高黄色素舍量)和Psy-A1b(低黄色素含量),各占50.0%,没有发现含Psy-A1c等位变异的品种(系);在Psy-B1位点,陕西小麦品种(系)内存在3种等住变异,其中以Psy-B1b(低黄色素含量)为主(65.3%),Psy-B1a(中等黄色素含量)次之(27.3%),Psy-B1c(高黄色素含量)较少(7.4%),没有发现舍Psy-B1d等位变异的品种(系).不同变异组合类型的平均分布比例表现不同,Psy-A1b/Psy-B1b组合类型所占的比例最高,为39.2%;其次是Psy-A1a/Psy-B1b组合类型,为26.1%;组合Psy-A1a/Psy-B1a(17.6%)、Psy-A1b/Psy-B1a(9.7%)和Psy-A1a/Psy-B1c(6.3%)比例较低,Psy-A1b/Psy-B1c(1.1 %)所占比例最低.不同地区不同等位变异及其组合类型的分布比例不同.总体来看,陕西小麦品种(系)中低黄色素含量的等住变异类型所占的比例较高.陕西不同地区育种目标和种植要求的差异,造成了不同地区小麦品种黄色素含量基因组成的不同.本研究所用分子标记扩增出的PCR奈带清晰,且稳定性好,可作为小麦黄色素含量分子标记辅助选择的有效工具.

关 键 词:陕西省  小麦  黄色素含量  分布频率

Detection and Distribution of Allelic Variations of Genes for Grain Yellow Pigment Content in Shaanxi Wheat Cultivars (lines)
ZHANG Ying quan,FU Xiao jie,ZHANG Xiao ke,ZHANG Xiao hui,ZHANG Tong xing,REN Wan jie,WEI Qian.Detection and Distribution of Allelic Variations of Genes for Grain Yellow Pigment Content in Shaanxi Wheat Cultivars (lines)[J].Journal of Triticeae Crops,2010,30(3):421-426.
Authors:ZHANG Ying quan  FU Xiao jie  ZHANG Xiao ke  ZHANG Xiao hui  ZHANG Tong xing  REN Wan jie  WEI Qian
Abstract:In order to investigate the distribution of allelic variations for genes Psy A1 and Psy B1 controlling wheat yellow pigment content on chromosomes 7A and 7B in Shaanxi wheat cultivars (lines). Functional molecular markers YP7A, YP7A 2, YP7B 1, YP7B 2 and YP7B 3 were used to detect 176 wheat cultivars (lines) released in Shaanxi province. The results showed that two allelic variations at Psy A1 locus were found in Shaanxi wheat cultivars (lines), Psy A1a and Psy A1b alleles with high and low YP content, respectively. The frequencies of both Psy A1a and Psy A1b alleles were 50.0%, and Psy A1c allele was absent in Shaanxi wheats. Three alleles Psy B1a, Psy B1b and Psy B1c at Psy B1 locus were found in Shaanxi wheat cultivars (lines). The frequencies of alleles Psy B1b, Psy B1a and Psy B1c were 65.3%, 27.3% and 7.4%, respectively. But Psy B1d allele was absent in Shaanxi wheats. Mean frequencies among allelic combinations in Shaanxi wheats were also different. The frequencies of allelic combinations Psy A1b/Psy B1b, Psy A1a/PsyB1b, Psy A1a/Psy B1a, Psy A1b/Psy B1a, Psy A1a/Psy B1c and Psy A1b/Psy B1c were 39.2%, 26.1%, 17.6%, 9.7%, 6.3% and 1.1%, respectively. The frequencies of those genotypes were also different among wheat cultivars (lines) from different regions of Shaanxi. Generally, number of cultivars with low YP content in Shaanxi province was slightly higher. This would be conducive to the selection of lower YP content cultivars in wheat quality breeding. The distribution of genotypes for YP content in different wheat regions of Shaanxi province was largely determined by their breeding target and planting requirement. The results showed that these functional markers used in this study could accurately identify the alleles of genes Psy A1 and Psy B1. They could be used as an effective tool for marker assisted selection (MAS) in wheat breeding programs targeting for either lower or higher YP content.
Keywords:Psy-A1  Psy-B1
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