| 小麦类病斑突变基因lm3的定位 |
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| 引用本文： | 杨佳秀,耿皆飞,李倩倩,刘录祥,谢彦周,王成社.小麦类病斑突变基因lm3的定位[J].麦类作物学报,2016,36(12):1578-1586. |
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| 作者姓名： | 杨佳秀 耿皆飞 李倩倩 刘录祥 谢彦周 王成社 |
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| 作者单位： | 1. 西北农林科技大学农学院,陕西杨陵,712100;2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 国家重点研发计划项目（2016YFD0102101）；中央高校基本科研业务费专项资金项目（2014YB081）；陕西省科技统筹创新工程计划项目（2014KTZB02-01-01，2015KTZDNY01-01-02）；西北农林科技大学南阳小麦试验示范站建设项目；西北农林科技大学唐仲英育种基金项目 |
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| 摘 要： | 为进一步对类病斑突变基因lm3进行克隆和功能研究,以EMS诱变普通小麦花培品系H261所得后代中选育出的一个稳定遗传的小麦类病斑突变体LF2010为研究材料,通过BSA(bulked segregant analysis)、MBSA(multiple bulked segregant analysis)、小麦90K基因芯片结合BSA三种不同的基因定位策略对该突变基因进行定位。结果表明,lm3基因位于小麦6B染色体的长臂,与其两侧标记SWES2和Xbarc134的遗传距离分别为13.1cM和3.8cM,为一个新的小麦类病斑突变基因。
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| 关 键 词： | 小麦 类病斑突变 基因定位 lm3 |
Mapping of Lesion Mimic Mutant Gene lm3 in Common Wheat |
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| YANG Jiaxiu,GENG Jiefei,LI Qianqian,LIU Luxiang,XIE Yanzhou,WANG Chengshe.Mapping of Lesion Mimic Mutant Gene lm3 in Common Wheat[J].Journal of Triticeae Crops,2016,36(12):1578-1586. |
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| Authors: | YANG Jiaxiu GENG Jiefei LI Qianqian LIU Luxiang XIE Yanzhou WANG Chengshe |
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| Abstract: | In order to clone the lesion mimic mutant gene lm3 and analyse its function further,a lesion mimic wheat mutant LF2010,obtained from an anther culture line H261 which was mutated with EMS,was chosen as material,and three kinds of different gene mapping strategies were taken to map it,including BSA(bulked segregant analysis),MBSA(multiple bulked segregant analysis),and wheat 90K gene chip combined with BSA.The result showed that a genetic linkage map containing 15 pairs markers linked with lm3 was obtained. Finally, lm3 was mapped on 6BL of wheat chromosome,13.8 cM away from marker SWES2 and 3.8 cM away from Xbarc134. lm3 is a new gene of wheat lesion mimic mutant. |
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| Keywords: | Wheat Lesion mimic mutant Gene localization lm3 |
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