大麦EMS突变体库构建和突变体筛选

为研究大麦重要功能基因并给大麦遗传育种提供新的种质资源,通过甲基磺酰乙酯(EMS)诱变处理优质、高抗黄花叶病大麦品种苏啤6号,从2400个M_2代株系中筛选出152个突变株系,突变频率为6.33%。其中,幼苗习性突变株系36个,突变率为1.50%;叶部突变株系34个,突变率为1.42%;茎部突变株系33个,突变体率为1.38%;穗部突变株系22个,突变率为0.92%;成熟期和育性突变株系27个,突变率为1.13%。经过M_3代验证,获得可稳定遗传的各类突变株系43个。

Construction of EMS-Treated Mutant Library and Mutant Phenotypic Analysis in Barley

In order to provide basic materials for studying the functional genomics and genetic breeding of barley,Supi 6 with a high quality and resistance to yellow mosaic disease was treated with the chemical mutagen ethyl methane sulfonate(EMS). A total of 152 mutants with significantly visible phenotypes were screened out from the 2 400 M₂ generation,with a mutation frequency of 6.33%. Of them,36 lines varied in seedling habits,with a mutation frequency of 1.50%; 34 lines varied in leaf,with a mutation frequency of 1.42%; 33 lines varied in stalk,with a mutation frequency of 1.38%; 22 lines varied in spike,with a mutation frequency of 0.92%; 27 lines varied in maturation stage and fertility,with a mutation frequency of 1.13%. In total,43 stably inherited lines were obtained by further agronomic trait investigation in M₃ generation.