Efficacy of entomopathogenic nematodes (Nemata: Rhabditida) to control Brazilian apple leafroller Bonagota salubricola (Meyrick, 1937) (Lepidoptera: Tortricidae) |
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Authors: | CRC Barbosa-Negrisoli AS Negrisoli Jr C Dolinski D Bernardi |
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Institution: | 1. Laboratório de Biologia de Insetos e Controle Biológico, FAEM/UFPel, 354, 96010-900, Pelotas, Brazil;2. Laboratório de Proteção de Plantas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos de Goytacazes, Brazil |
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Abstract: | The Brazilian apple leafroller, Bonagota salubricola (Meyrick, 1937) (Bonagota cranaodes) (Lepidoptera: Tortricidae), is one of the main pest problems in apple orchards is Brazil. The purpose of this work was to evaluate B. salubricola larval and pupal susceptibility to different species of entomopathogenic nematodes, isolated in Rio Grande do Sul state in Brazil, under laboratory and field (apple orchard) conditions. Bioassays for isolates selection and determination of lethal concentration were performed in tubes of 1.5 ml (eppendorf), each containing one B. salubricola third instar larvae and filter paper. Field experiments were performed in commercial orchard, with application of 100 infecting juveniles (IJs)/cm2 for each apple plant previously infected with five B. salubricola larvae covered with plastic trays containing thin cloth. Nematodes H. bacteriophora RS107 and H. bacteriophora RS57 had LC50 of 13 and 4.5 IJs/larvae (4.3 and 1.5 IJs/cm2), respectively. In the field, H. bacteriophora RS107 and H. bacteriophora RS57, applied with sorbitol as an adjuvant, reached 70.2 and 61.1% larval mortality, respectively. The results showed that both isolates had biological activity against B. salubricola under laboratory and field conditions. |
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Keywords: | Biological control Heterorhabditis Steinernema Tortricidae Brazilian apple leafroller Apple |
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