Efficient shoot regeneration from direct apical meristem tissue to produce virus-free purple passion fruit plants |
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Authors: | Siripatr Prammanee Sarut Thumjamras Pissawan ChiemsombatNarongchai Pipattanawong |
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Institution: | a Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand b Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok 10900, Thailand c Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand d Agro-Ecological System Research and Development Institute, Kasetsart University, Bangkok 10900, Thailand |
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Abstract: | Purple passion fruit is an important fresh table fruit. At present, the production of passion fruit is decreasing because of the spread of viral diseases throughout the planting area. The aim of this research was to propagate virus-free plants using a tissue culture technique involving the apical meristem of purple passion fruit. Shoot tips were excised to a length of 2 mm and the shoots were regenerated by culturing on Murashige and Skoog medium supplemented with different concentrations of benzyladenine (BA) (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l) and 1-naphthaleneacetic acid (NAA) (0.2, 0.5, 1.0, 1.5 and 2.0 mg/l). Root formation was promoted using different concentrations of indole-3-butyric acid (IBA) (0.0, 0.2, 0.4, 0.6, 0.8 and 1.2 mg/l). A greater number of shoots were produced with BA concentrations of 1.0 and 1.5 mg/l than with any other BA concentrations tested (less than 1.0 mg/l or greater than 1.5 mg/l). However, when NAA at any concentration was included in the medium, no shoots were produced in culture. The cultures including 1.0 mg/l and 1.5 mg/l BA were then subcultured four times every two weeks. Initially, the tissue cultured in the 1.5 mg/l BA medium grew faster than that cultured in the 1.0 mg/l BA medium. The tissue cultured with 1.5 mg/l BA generated many short shoots, whereas the tissue cultured with 1.0 mg/l BA, generated long shoots that could be subcultured into individual plants. These regenerated shoots were assayed for the presence of the passion fruit woodiness virus using ELISA or a test strip kit; only virus-free shoots were used for further propagation. Root formation was very good in IBA concentrations of 0.4 and 0.6 mg/l. Thus, virus-free plants could be successfully regenerated directly from the apical meristem. |
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Keywords: | Apical meristem culture Purple passion fruit Virus-free plant Shoots regeneration Root formation |
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