Incidence of Cymbidium mosaic virus and Odontoglossum ringspot virus affecting Oncidium orchids in Hainan Island,China |
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| Institution: | 1. Post-Entry Quarantine Center for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou 570311, China;2. College of Environment and Plant Protection, Hainan University, Haikou 570228, China;1. Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China;2. First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China;1. Warsaw University of Life Sciences (SGGW), Faculty of Agriculture and Biology, Department of Botany, Nowoursynowska 159, Building 37, 02-776 Warsaw, Poland;2. University of Agriculture, Faculty of Biotechnology and Horticulture, Institute of Plant Biology and Biotechnology, Unit of Botany and Plant Physiology, Al. 29-Listopada 54, 31-425 Krakow, Poland;3. University of Agriculture, Faculty of Agriculture and Economics, Institute of Soil Science and Agrophysics, Department of Soil Science and Soil Protection, Al. Mickiewicza 21, 31-120 Krakow, Poland;4. University of Agriculture, Center for Technology Transfer, Al. Mickiewicza 21, 31-120 Krakow, Poland;1. Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456, USA;2. Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 12 Rue de Général Zimmer, 67084 Strasbourg, France;3. SVQV, Université de Strasbourg, INRA, 68000 Colmar, France;1. Department of Chemistry, R.V. College of Engineering, Bangalore, India;2. Department of Biotechnology, R.V. College of Engineering, Bangalore, India;1. Department of Anatomy & Neurobiology, University of Kentucky, Lexington, KY, 40536-0298, USA;2. Naprogenix Inc, University of Kentucky, AsTeCC, Lexington, KY 40506-0286, USA;3. Department of Psychology, University of Kentucky, Lexington, KY, 40506-0044, USA;1. Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China;2. Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China |
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| Abstract: | Oncidium is the most popular cut flower for the orchid industry in Hainan Island and several viruses have been reported to negatively impact their growth and yield. A survey of Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) in Oncidium orchids cultivated in Hainan Island was conducted during 2009–2012 with indirect enzyme-linked immunosorbent assay (ELISA). A total of 171,585 samples from adult plant, tissue-culture flask, seedling, and medium seedling were tested for CymMV and ORSV. Of the 24,125 adult plant samples from eighteen commercial orchid farms, 77.58% were infected with CymMV, 16.04% with ORSV, and 10.19% with both CymMV and ORSV. Of the 67,080 samples cultivated in tissue-culture flasks from eight tissue culture laboratories, 41.18% were infected with CymMV, 6.11% with ORSV, and 4.46% with both CymMV and ORSV. Of the 54,450 seedling samples from eight seedling nurseries, 53.20% were infected with CymMV, 9.00% with ORSV, and 5.23% with both CymMV and ORSV. And of the other 25,930 medium seedling samples from twelve medium seedling nurseries, 44.96% were infected with CymMV, 7.90% with ORSV, and 5.15% with both CymMV and ORSV. Plant material must be tested for the presence of viruses before being used for mass production by tissue culture, and sanitation during propagation of orchid plants and harvesting of flowers is essential in order to prevent the virus from spreading. These findings emphasize the need to implement a virus-free certification programme that would greatly enhance the management of these two prevalent viruses in the Hainan orchid industry. |
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| Keywords: | Incidence ELISA |
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