Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79 |
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| Institution: | 1. College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shanxi 712100, PR China;2. College of Agronomy, Yangtze University, Jingzhou, Hubei 434025, PR China;3. Shaanxi Tobacco Research Institute, Xi''an, Shanxi 710003, PR China;4. Qingzhou Tobacco Research Institute of China National Tobacco Crop, Qingdao 266001, China;1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, People''s Republic of China;2. Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, People''s Republic of China;3. Zhejiang Key Laboratory for Agro-food Processing, Zhejiang University, Hangzhou 310058, People''s Republic of China;1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;2. Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, China;3. Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian 223003, China;4. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Agriculture, Nanjing 210095, Jiangsu, China;2. Central Agricultural Research Institute, Suakoko, Bong County, Liberia;1. Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education and Jiangsu Province, Institute of Agricultural Engineering of Jiangsu University, 212013 Zhenjiang, China;2. School of Environment, Jiangsu University, 212013 Zhenjiang, China;3. School of Mechanics and Power Engineering, Henan Polytechnic University, Jiaozuo 454003, China;4. College of Forest Resources and Environment, Nanjing Forestry University, 210037 Nanjing, China;1. Key Laboratory of Agricultural Biodiversity for Plant Disease Management Under the Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China;2. Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722, USA;3. Agricultural-based Experimental Center, YAU, Kunming, Yunnan 650201, PR China;4. College of Bioengineering, Sichuan University of Science and Engineering, Zigong, Sichuan 643100, PR China |
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| Abstract: | Strain BC79, isolated from primeval forest soil in Qinling, Mountains, China, was identified as Bacillus methylotrophicus based on morphological, biochemical, physiological and chemotaxonomic analyses as well as phylogenetic 16S rDNA sequencing data. This strain was able to suppress mycelial growth and conidial germination of numerous plant pathogenic fungi in dual cultures on solid media. For exploring potential biocontrol activity, we assessed fermentation conditions for studying B. meth1ylotrophicus BC79. The active substance of BC79, phenaminomethylacetic acid, was extracted by TLC and HPLC, and identified as the strongest inhibitory substance described in B. methylotrophicus. Experiments in a greenhouse showed that application of BC79 culture filtrates 24 h before inoculation of Magnaporthe oryzae, the causal agent of rice blast, had 89.87% biocontrol efficiency. B. methylotrophicus BC79 colonized rice plant tissues and at 10 days after filtrate application, its population in leaves (1.65 × 108 CFU/g) was much larger than in stems (6.78 × 107 CFU/g) or roots (3.56 × 107 CFU/g). Field trials indicated that BC79 culture filtrate (4000 g/667 m2) showed the highest efficiency for M. oryzae, with 84.8% biocontrol effect, followed by of 15% phenaminomethylacetic acid extract (75.5%) and 20% tricyclazole (76.1%). Seedling and post-transplant stages were the best periods to apply BC79 for control of rice blast. The B. methylotrophicus BC79 strain hence has enormous potential as an agricultural agent for biocontrol of rice blast. |
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