高效液相色谱柱后光化学反应-荧光检测茶叶中黄曲霉毒素B1

建立了高效液相色谱/光化学反应器/荧光检测器测定茶叶中黄曲霉毒素B1的方法。用乙腈水溶液(V∶V=86∶14)提取黄曲霉毒素B1,提取液经净化柱和黄曲霉毒素B1免疫亲和柱净化,高效液相色谱测定。在黄曲霉毒素B1标准溶液质量浓度为0.591~5.91μg/L时,峰面积与浓度呈现良好的线性关系,黄曲霉毒素B1的回收率为85.4%~98.9%(添加量分别为0.510μg/kg、7.090μg/kg和14.180μg/kg),相对标准偏差为0.2%~1.8%,方法检出限为0.1μg/kg。运用所建立方法对市售的8个茶样及加标样品中的黄曲霉毒素B1进行检测,结果显示该方法选择性强、灵敏度高,适合茶叶中黄曲霉毒素B1的测定。

Determination of Aflatoxins B1 in Tea by High Performance Liquid Chromatography-Fluorescence Detector with Post-column Photochemical Reaction

A new method for the sensitive determination of Aflatoxins B1 in tea by high performance liquid chromatography with photoelectric reactor and fluorescence detector was established.A solution of V(acetonitrile)∶ V(H2O)=86∶ 14 was used to extract Aflatoxins B1 from tea.The extracted solution was then purified by a multifunctional and immuneaffinity column,respectively.The peak area and the concentration of Aflatoxins B1 showed a good linear relationship within the range from 0.591 μg/L to 5.91 μg/L with a linear correlation coefficient(r) of 0.9994.The recoveries at the concentrations studied low level(7.090 μg/kg),high level(14.180 μg/kg)] were between 85.6% and 98.9% with a relative standard deviations ranging from 1.7% to 1.8%.The limit of detection(LOD) is 0.1 μg/kg(S/N=3).The limit of quantitation(LOQ) is(0.591 μg/kg).The new method was used to analyze eight tea samples collected from the local markets and negative results were obtained.The meth od is suitable for detection of Aflatoxins B1 in tea with high selectivity and sensitivity.