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| EGCG与锌离子互作对PC-3细胞能量代谢的影响 | |
| 引用本文： | 孙世利,凌彩金,于海宁,沈生荣,苗爱清,潘顺顺,庞式,卓敏,赵超艺.EGCG与锌离子互作对PC-3细胞能量代谢的影响[J].茶叶科学,2009,29(4). |
| 作者姓名： | 孙世利 凌彩金 于海宁 沈生荣 苗爱清 潘顺顺 庞式 卓敏 赵超艺 |
| 作者单位： | 1. 广东省农业科学院茶叶研究所,广东,广州,510640 2. 浙江工业大学药学院,浙江,杭州,310032 3. 浙江大学生物系统工程与食品科学学院,浙江,杭州,310029 |
| 摘 要： | 采用Hoechst33258荧光染色法观察EGCG、Zn2+和EGCG+Zn2+对前列腺癌PC-3细胞凋亡的诱导作用;采用HPLC法检测了EGCG、Zn2+和EGCG+Zn2+对PC-3细胞内腺苷酸(ATP、ADP、AMP)含量的影响,并分析了能量负荷(EC)和ATP/ADP比值。结果表明,Hoechst33258染色后正常细胞发出均匀微弱的蓝色荧光,细胞核未见异常,EGCG、Zn2+和EGCG+Zn2+处理后凋亡的PC-3细胞都发出较强的蓝色荧光,细胞核DNA断裂及染色体高度浓缩;EGCG、Zn2+及二者混合物处理后细胞内总腺苷酸含量、EC及ATP/ADP比值都下降,表明EGCG与Zn2+对PC-3细胞能量代谢都存在明显的抑制作用。 |
| 关 键 词： | EGCG 锌离子 PC-3细胞 细胞凋亡 腺苷酸 能量代谢 |
Effect of Interactions Between EGCG and Zn2+ on Energy Metabolism of PC-3 Cells |
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| SUN Shi-li,LING Cai-jin,YU Hai-ning,SHEN Sheng-rong,MIAO Ai-qing,PAN Shun-shun,PANG Shi,ZHUO Min,ZHAO Chao-yi.Effect of Interactions Between EGCG and Zn2+ on Energy Metabolism of PC-3 Cells[J].Journal of Tea Science,2009,29(4). | |
| Authors: | SUN Shi-li LING Cai-jin YU Hai-ning SHEN Sheng-rong MIAO Ai-qing PAN Shun-shun PANG Shi ZHUO Min ZHAO Chao-yi |
| Institution: | 1. Tea Research Institute Guangdong Academy of Agricultural Sciences;Guangzhou 510640;China;2. College of Pharmaceutical Sciences;Zhejiang University of Technology;Hangzhou 310032;3. Department of Food Science and Nutrition;Zhejiang University;Hangzhou 310029;China |
| Abstract: | In the present work,the effect of treatments with EGCG,Zn2+ and EGCG+Zn2+ on inducing apoptosis and content of adenylate nucleotide (ATP,ADP and AMP) in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC.Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 μmol/L EGCG,80 μmol/L Zn2+ and 80 μmol/L EGCG+80 μmol/L Zn2+ in PC-3 cells.In the determination of adenylate nucleotide,after treated with EGCG,Zn2+ and EGCG+Zn2+,content of total adenylate nucleotide,EC and ATP/ADP value were significantly decreased in PC-3 cells,which demonstrated that EGCG and Zn2+ have a markedly inhibitory effect on energy metabolism of PC-3 cells. |
| Keywords: | EGCG Zn2+ PC-3 cells apoptosis adenosine triphosphate energy metabolism |
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