| 茶树蛋白质双向电泳样品制备技术研究 |
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| 引用本文： | 李勤,黄建安,刘硕谦,李娟,刘仲华.茶树蛋白质双向电泳样品制备技术研究[J].茶叶科学,2011,31(3):173-178. |
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| 作者姓名： | 李勤 黄建安 刘硕谦 李娟 刘仲华 |
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| 作者单位： | 1. 国家植物功能成分利用工程技术研究中心,湖南 长沙 410128; 2. 茶学教育部重点实验室,湖南 长沙 410128 |
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| 基金项目： | 国家自然科学基金“茶氨酸基因合成的基因转录调控研究”(30871572) |
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| 摘 要： | 为探索一种适用于茶树蛋白质双向电泳的样品制备方法,研究比较了TCA/丙酮沉淀法、改良的Tris-HCl抽提法,以及酚-甲醇/醋酸铵沉淀法3种植物蛋白质样品制备方法。结果表明,改良的Tris-HCl抽提法较适合于茶树蛋白质双向电泳的样品制备。该改良方法制备的茶树蛋白质样品经双向电泳分离,可获得1117±9.89个蛋白质点,其中大部分蛋白质的等电点集中在pI5~7之间,相对分子质量集中在15.00~95.00kD之间,背景清晰且蛋白质得率较高,能满足茶树蛋白质组学研究的需要。
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| 关 键 词： | 茶树 双向电泳 蛋白质样品制备 蛋白质组学 |
| 收稿时间： | 2011-01-10 |
Protein Extraction Method Suitable for Tea Plant |
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| LI Qin,HUANG Jian-an,LIU Shuo-qian,LI Juan,LIU Zhong-hua.Protein Extraction Method Suitable for Tea Plant[J].Journal of Tea Science,2011,31(3):173-178. |
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| Authors: | LI Qin HUANG Jian-an LIU Shuo-qian LI Juan LIU Zhong-hua |
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| Institution: | 1. National Research Center of Engineering Technology for Utilization of Functional Ingredients From Botanicals, Changsha 410128, China; 2. Key Lab of Tea Science, Ministry of Education, P.R. C., Changsha 410128, China |
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| Abstract: | In order to develop an efficient protein extraction method suitable for two-dimensional electrophoresis from tea plant,three methods(TCA/acetone precipitation,improved Tris-HCl extraction and phenol-methanol/ammonium acetate precipitation) for total protein extraction in plant were compared.The result showed that the improved Tris-HCl extraction was the best method suitable for tea leaf protein sample preparation in these three techniques and the extracts were separated by two-dimensional electrophoresis.Co... |
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| Keywords: | Camellia sinensis(L ) O Kuntze two dimensional electrophoresis protein sample preparation proteomics |
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