| EGCG氧化产物不同级分的分析及其抗氧化活性的研究 |
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| 引用本文： | 戚向阳.EGCG氧化产物不同级分的分析及其抗氧化活性的研究[J].茶叶科学,2008,28(6). |
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| 作者姓名： | 戚向阳 |
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| 作者单位： | 浙江万里学院生物与环境学院,浙江,宁波,315100;华中农业大学食品科技学院,湖北,武汉,430070 |
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| 基金项目： | 国家十一五重点支撑项目 |
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| 摘 要： | 利用乙酸乙酯在中性和酸性条件下萃取EGCG氧化产物,得到级分A(EOPA)和级分B(EOPB),用化学发光法比较EGCG与EOPA、EOPB的抗氧化活性及其对DNA损伤的保护作用,并通过HPLC-MS分析两级分中的氧化产物。结果表明EGCG及EOPA、EOPB均有较好的清除活性氧和保护DNA损伤的活性,其中EOPA的活性强于未氧化的EGCG。HPLC-MS分析表明,EOPA、EOPB级分中的氧化产物均为二聚体。
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| 关 键 词： | EGCG氧化产物 化学发光 抗氧化 HPLC-ESI-MS |
Study on the Analysis and Antioxidant Activities of Different Extracts from (-)-Epigallocatechin Gallate Oxidation Products |
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| QI Xiang-yang.Study on the Analysis and Antioxidant Activities of Different Extracts from (-)-Epigallocatechin Gallate Oxidation Products[J].Journal of Tea Science,2008,28(6). |
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| Authors: | QI Xiang-yang |
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| Institution: | QI Xiang-yang1,2 (1. College of Biological Environmental Sciences,Zhejiang Wali College,Ningbo 315100,China,2. Department of food science , technology of Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | (–)-Epigallocatechin gallate (EGCG) was oxidized chemically and extracted under neutral, acidic condition value to obtain two extracts: fraction A (EOPA) and fraction B (EOPB). The antioxidant activities of EOP A, EOP B and EGCG were determined using a chemiluminescence technique. The components of two fractions were analyzed by HPLC-ESI-MS. Results showed that the EOP A possessed the strongest antioxidant activity in all test system followed by EGCG, EOP B in a decreasing order. HPLC-ESI-MS analysis showed that the oxidation products of EOP A and EOPB were all dimmers. |
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| Keywords: | HPLC-ESI-MS |
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