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ISSR分子标记在茶树遗传关系分析中的初步应用
引用本文:林郑和,陈荣冰,陈常颂,林金科,郝志龙,高水练,陈梁城.ISSR分子标记在茶树遗传关系分析中的初步应用[J].茶叶科学,2007,27(1):45-50,60.
作者姓名:林郑和  陈荣冰  陈常颂  林金科  郝志龙  高水练  陈梁城
作者单位:1. 福建省农业科学院茶叶研究所,福建,福安,355015
2. 福建农林大学园艺学院,福建,福州,350002
基金项目:福建省农业科学院重点学科建设B类项目
摘    要:ISSR标记是一种基于微卫星序列发展起来的十分有效的分子标记。本文主要利用ISSR分子标记对我国39份茶树种质资源进行遗传关系研究,从40条引物中筛选出15条引物,共扩增出143个位点,其中多态性位点为131个,占91.6%。通过UPGMA聚类分析,39个茶树种质资源GS变化范围0.21~0.95,其中慢奇兰与竹叶奇兰GS值最大、遗传相似程度最高,遗传距离最近;崇庆枇杷茶与英红1号GS值最小、遗传相似程度最低、遗传学差异最大。聚类分析将39个种质资源划分为3个大类(GS=0.20),崇庆枇杷与英红1号,属于原始类型资源,归为一类;九龙珠与黄龙,属于较原始类型,也归为一类;其余的种质资源归为一类。据此认为,ISSR作为一种信息量高、重演性好的分子标记,应用于茶树遗传多样性和亲缘关系分析是非常有效。

关 键 词:ISSR  遗传多样性  亲缘关系  茶树
文章编号:1000-369X(2007)01-045-06
收稿时间:2006-06-28
修稿时间:2006-06-282006-11-21

Preliminary Application of ISSR Markers in the Genetic Relationship Analysis of Tea Plants
LIN Zheng-he,CHEN Rong-bing,CHEN Chang-song,LIN Jin-ke,HAO Zhi-long,GAO Shui-lian,CHEN Lian-cheng.Preliminary Application of ISSR Markers in the Genetic Relationship Analysis of Tea Plants[J].Journal of Tea Science,2007,27(1):45-50,60.
Authors:LIN Zheng-he  CHEN Rong-bing  CHEN Chang-song  LIN Jin-ke  HAO Zhi-long  GAO Shui-lian  CHEN Lian-cheng
Abstract:Inter-simple sequence repeat (ISSR) is a kind of effective molecular marker developed on the basis of microsatellite, or simple sequence repeat (SSR). ISSR markers were applied to detect the relationship of the thirty-nine cultivars of tea plants. Fifteen primers, selected from fourty primers, were used to amplify the tea samples one hundred and fourty-three DNA bands were amplified, of which one hundred and thirty-one bands were polymorphic.The percentage of polymorphic bands was 91.6%.By cluster analysis based on ISSR markers using UPGMA,the genetic similarity was from 0.21 to 0.95. The GS was the greatest between Manqilan and Zhuyeqilan, and they had the highest genetic similarity and the smallest genetic distance.The GS between Chongqinpipacha and Yinghong 1 was the smallest and they had the lowest genetic similarity and the greatest genetic difference. Cluster analysis classified the 39 cultivars into 3 major groups (GS=0.20). Chongqinpipacha and Yinghong 1 fell into one group, belong to primitive form Jiulongzhu and Huanglong also fell into one group. The rest formed the third group. The results suggest that ISSR is a useful tool for molecular genetic diversity and relationship analysis of tea cultivars.
Keywords:ISSR  genetic diversity  relationship  tea plants
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