| EGCG甲基化衍生物酶促合成的反应条件研究 |
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| 引用本文： | 吕海鹏,费冬梅,张悦,林智,彭群华,郭丽,谭俊峰.EGCG甲基化衍生物酶促合成的反应条件研究[J].茶叶科学,2012,32(5):441-447. |
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| 作者姓名： | 吕海鹏 费冬梅 张悦 林智 彭群华 郭丽 谭俊峰 |
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| 作者单位： | 国家茶产业工程技术研究中心,中国农业科学院茶叶研究所,浙江杭州310008 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项，国家自然科学基金项目，现代农业产业技术体系建设专项资金资助 |
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| 摘 要： | 以EGCG-O-甲基转移酶催化EGCG生成EGCG3′′Me、EGCG4′′Me和EGCG3′Me等3种主要的EGCG甲基化衍生物的生成量为主要指标,考察了EGCG甲基化衍生物酶促合成的反应条件。结果表明,EGCG甲基化衍生物的酶促合成最佳反应条件为:温度35℃,pH7.5,DTT和Mg2+的浓度为2 mmol/L;在该反应条件下,反应体系中EGCG3′′Me、EGCG4′′Me以及EGCG3′Me的生成量分别可达到386.39μg/mL、23.40μg/mL和107.01μg/mL。
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| 关 键 词： | EGCG甲基化衍生物 酶促合成 EGCG-O-甲基转移酶 |
Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase |
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| LV Hai-peng
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FEI Dong-mei
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ZHANG Yue
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LIN Zhi
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PENG Qun-hua
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GUO Li
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TAN Jun-feng.Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase[J].Journal of Tea Science,2012,32(5):441-447. |
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| Authors: | LV Hai-peng FEI Dong-mei ZHANG Yue LIN Zhi PENG Qun-hua GUO Li TAN Jun-feng |
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| Institution: | National Engineering Research Center for Tea Industry,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China |
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| Abstract: | Taken the yields of EGCG3"Me,EGCG4′′Me and EGCG3′Me as the main index,the investigation on the reaction condition of EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were conducted.Results showed that the optimum reaction condition was as follows: the concentration of DTT and Mg 2+ was 2 mmol/L,the initial pH was 7.5 and the reaction temperature was 35℃.Under the above conditions,the yields of EGCG3"Me,EGCG4′′Me and EGCG3′Me could reach 386.39 μg/mL,23.40 μg/mL and 107.01 μg/mL,respectively. |
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| Keywords: | EGCG methylated derivatives enzymatic synthesis EGCG-O-Methyltransferase |
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