| EGCG纳米粒的制备及其抗肿瘤活性研究 |
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| 引用本文： | 黄美蓉,应浩,江用文,江和源,杜琪珍.EGCG纳米粒的制备及其抗肿瘤活性研究[J].茶叶科学,2015,35(6):605-612. |
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| 作者姓名： | 黄美蓉 应浩 江用文 江和源 杜琪珍 |
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| 作者单位： | 1. 浙江工商大学食品与生物工程学院,浙江 杭州 310018; 2. 中国农业科学院茶叶研究所,浙江 杭州 310008 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划项目 |
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| 摘 要： | 采用热诱导法制备表没食子儿茶素没食子酸酯-抗坏血酸-β-乳球蛋白纳米粒（EGCG-Vc-β-Lg）,考察EGCG与Vc摩尔比对纳米粒粒径、Zeta电位、包埋率、装载量、颜色变化的影响;运用噻唑蓝（MTT）比色法和胞质分裂阻滞微核试验（CBMNT）研究纳米粒对人体黑色素瘤细胞（A-375）、食管癌细胞（TE-1）的增殖抑制作用和初步作用机制。结果表明：EGCG-Vc-β-Lg对A-375、TE-1的增殖抑制有增效作用,并且显著提高了其微核率、凋亡率和坏死率。
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| 关 键 词： | 表没食子儿茶素没食子酸酯 抗坏血酸 β-乳球蛋白 纳米粒 抗肿瘤 |
| 收稿时间： | 2015-04-08 |
Research on Preparation and Antitumor Activity of EGCG Naonparticles |
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| HUANG Meirong,YING Hao,JIANG Yongwen,JIANG Heyuan,DU Qizhen.Research on Preparation and Antitumor Activity of EGCG Naonparticles[J].Journal of Tea Science,2015,35(6):605-612. |
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| Authors: | HUANG Meirong YING Hao JIANG Yongwen JIANG Heyuan DU Qizhen |
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| Institution: | 1. The Institute of Food and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; 2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China |
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| Abstract: | (-)-Epigallocatechin-3-gallate(EGCG) and ascorbic acid(Vc) were loaded in heat treatedβ-lactoglobulin (β-Lg) to prepare EGCG naonparticles. The effects of EGCGand Vcwith different molar ratioson the particle size, Zeta potential,entrapment efficiency,loading capacity,the change of color were investigated. The antitumor activity and mechanism of EGCG naonparticles were obtained by MTT assay and cytokinesis block micronucleus test (CBMNT). The results showed that EGCG naonparticles had stronger inhibition on human melanoma cell(A-375) and esophageal carcinoma cell(TE-1), and significantly increased the number of micronuclei, necrosis and apoptosis of the A-375 and TE1-1 carcinoma cell. |
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| Keywords: | (-)-epigallocatechin-3-gallate (EGCG) Vc β-lactoglobulin(β-Lg) nanoparticle antitumor activity |
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