普洱茶发酵过程中外源接种黄曲霉产毒研究

以云南大叶种晒青茶为原料,接种产毒黄曲霉进行模拟普洱茶发酵试验,并在发酵结束时通过LC-MS/MS法对茶样进行黄曲霉毒素检测,研究普洱茶发酵过程中黄曲霉的生长及产毒情况。结果表明,在普洱茶发酵过程中,接种的黄曲霉能在茶叶中生长繁殖,初期生长较快,但随发酵时间的延长,黄曲霉在茶叶中的生长明显受到抑制,其数量逐渐下降。发酵终止时,未在茶样中检测出黄曲霉毒素。

Research on Aspergillus flavus Toxin-producing in Pu'er Tea Fermentation

For the purpose of studying the Aspergillus flavus growth and toxin production in Pu′er tea fermentation, the Yunnan large-leaves solar-drying green tea was used as the raw material to fermentate by inoculating toxigenic Aspergillus flavus fungus in Pu′er tea. Aflatoxin was tested by LC-MS/MS method at the end of tea samples fermentation. The results showed that during the tea fermentation, Aspergillus flavus growth was observed in the initial fermentation. However, with the extension of Pu′er tea fermentation time, Aspergillus flavus growth were inhibited in the late fermentation, the number decreased gradually, while the end of fermentation, the aflatoxin was not detected in tea samples.