甘蓝型油菜BnTLP1基因的菌核病抗性研究

前期发现甘蓝型油菜类甜蛋白基因BnTLP1在菌核病抗性品种中的表达丰度高，为探究和解析其作用机制，通过叶片接种核盘菌，对拟南芥BnTLP1过表达转基因株系、野生型对照和突变体tlp1-1之间的抗菌核病效果进行分析。结果表明：过表达BnTLP1拟南芥的病斑面积显著小于野生型对照，而tlp1-1病斑扩展大于对照，即BnTLP1正调控菌核病抗性。亚细胞定位表明BnTLP1定位于细胞膜和细胞核，暗示其不仅在细胞膜发挥抑菌功能，而且在核内行使其它功能。不同防御反应信号通路的标志基因表达分析表明，茉莉酸/乙烯途径可能在早期参与了BnTLP1介导的菌核病抗性。

BnTLP1 on resistance to Sclerotinia sclerotiorum in Brassica napus

BnTLP1 gene was previously found highly expressed in tolerant Brassica napus cv. Zhongyou 821. To deep understand the tolerance to Sclerotinia sclerotiorum, BnTLP1 gene was identified using overexpressed transgenic Arabidopsis lines. Leaf inoculation was carried out using BnTLP1 overexpression transgenic Arabidopsis lines, wild-type and tlp1-1 mutant by S. sclerotiorum mycelial agar plugs. Compared to WT, BnTLP1 overexpression lines exhibited the smallest lesion, while tlp1-1 showed the largest one, suggesting that BnTLP1 could positively regulated Sclerotinia disease resistance. Subcellular localization indicated that BnTLP1 was located in cell membrane and nucleus. Expression analysis of marker genes in different defense signaling showed that jasmonic acid/ethylene might play roles in the early stage of BnTLP1-mediated diseased resistance.