野生大豆ZYD00006回交导入系构建

为挖掘野生大豆优异稀有基因,2006年至今以栽培大豆绥农14（轮回亲本）与野生大豆ZYD00006（供体 亲本）为双亲材料,经杂交、回交,标记辅助选择构建获得一套覆盖野生大豆全基因组的染色体片段导入系（代换 系）。该群体共 192个株系,包含野生大豆目标导入片段 237个,平均每个连锁群的导入片段个数为 11.85个;导入 片段总长度1865.17 cM,覆盖整个基因组的82.43%。其中L连锁群野生大豆基因组覆盖率最高,为100%,N连锁群 覆盖最低为 53.17%。最长导入片段 43.30 cM,最短导入片段 0.22 cM。高度一致的遗传背景对大豆重要基因及野 生大豆特有优异基因挖掘具有重要意义。同时,野生资源的引入极大丰富了栽培大豆的遗传基础,进而使得导入 系后代表型变异丰富,为大豆遗传育种提供重要的材料基础。

Construction of wild soybean backcross introgression lines

To excavate the excellent rare gene of wild soybean,this study had been carried out since 2006,Sui nong 14(recurrent parent)and wild soybean ZYD00006(donor parent)were used as parents to construct a set of chromosome fragment introduction lines(substitution lines)covering the whole genome of wild soybean through hy bridization,backcross and marker assisted selection.A total of 192 plant rows in this population,included 237 wild soybean introduction fragments,with the average number of introduction fragments per linkage group of 11.85.The total length of the introduction fragment was 1865.17cM with the coverage of 83.23%of wild soybean genome.The coverage rate of linkage group L was the highest of 100%;linkage group N had the least coverage,of 53.17%.The maximum length of segment was 43.30 cM and the minimum one was 0.22 cM.Highly consistent genetic back ground was of great significance for excavating important genes of soybean and wild soybean-specific excellent gene.At the same time,the introduction of wild resources greatly enriched the genetic basis of cultivated soybean,and further enriched the phenotypic variation of the introgression lines,providing an important material basis for soybean genetic breeding.