利用SRAP标记分析花生属花生区组种质亲缘关系

利用54对SRAP引物分析花生属花生区组11个物种28份材料间的遗传变异，结果表明， 28份材料共扩增出327条多态性DNA带，平均每对引物扩增出5.95条多态性DNA带，扩增变幅为2～25条。聚类分析表明，28份花生材料间的遗传距离变幅为0.12～0.75，平均为0.42，A基因组物种A. duranensis与栽培种花生的亲缘关系最近，可能是栽培种花生的A基因组的供体。28份种质分为两大类，第一大类包含四倍体种质及A基因组的A. villosa和A. duranensis，第二大类包含B基因组、D基因组及其他A基因组。主成分分析结果与聚类分析结果相似，仅将第二大类中的B基因组种质A.batizocoi独立作为第三大类；第一和第二个主成分可以解释81%（57.5%和23.5%）的总变异。

Genomic affinities of Arachis section Arachis revealed by SRAP markers

Fifty-four SRAP primers were selected to analyze genetic variability among 28 accessions of Arachis section from 11 species.A dendrogram and principal component analysis were constructed based on our data.The results showed that 327 SRAP polymorphic bands were acquired.The number of scored polymorphisms bands by each primer combination varied from 2 to 25 with an average of 5.95 per reaction.That indicated the availability of SRAP technique in studying genetic variability in Arachis.Estimates of genetic distance among the 28 accessions Arachis section ranged from 0.12 to 0.75 with average genetic distance of 0.42.A genetic distance clarified that A-genome A.duranensis was most closely associated with tetraploid germplasm,which could be A-genome donors.Dendrogram of cluster analysis classified 28 accessions Arachis section into two groups:one including tetraploid germplasm,and A.duranensis and A.villosa of A-genome;the other including the rest of A-genome,B and D-genome.Principal component analysis identified the analogical results,but classified that A.batizocoi of B-genome was unattached third kind.The first two PCAs accounted for 81% (57.5% and 23.5%) of the total variation observed.