| TDZ和BA对诱导大豆胚轴植株再生的影响 |
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| 引用本文： | 张晓娟,方小平,罗丽霞.TDZ和BA对诱导大豆胚轴植株再生的影响[J].中国油料作物学报,2000,22(1):24-26. |
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| 作者姓名： | 张晓娟 方小平 罗丽霞 |
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| 作者单位： | 中国农业科学院油料作物研究所!湖北武汉430062 |
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| 基金项目： | 教育部归国人员科技活动基金,农业部油料作物遗传改良重点实验课题! ( 970 0 3 ) |
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| 摘 要： | 大豆未成熟种子和萌动种子胚轴在含有ＴＤＺ和ＢＡ的培养基上,通过器官发生,可获得再生株。培养基中同时加入ＴＤＺ和ＢＡ〈能够增加不定芽的形成和再生株的数量,其中以２．０ｍｇ／ＬＢＡ＋０．７５ｍｇ／ＬＴＤＡ处理再生效率最高。诱导培养基中适当增加ＮＡＡ浓度有利于不定芽的分化。
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| 关 键 词： | 大豆 器官发生 TDZ 胚轴 BA 植株再生 |
| 修稿时间： | 1999-12-01 |
Influence
of TDZ and BA on efficiency of plant regeneration via organogenesis in soybean |
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| ZHANG Xiao juan,FANG Xiao ping,LUO Li xia,ZHOU Xin an,XU Ze yong.Influence
of TDZ and BA on efficiency of plant regeneration via organogenesis in soybean[J].Chinese Journal of Oil Crop Sciences,2000,22(1):24-26. |
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| Authors: | ZHANG Xiao juan FANG Xiao ping LUO Li xia ZHOU Xin an XU Ze yong |
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| Abstract: | The embryonic axes of immature seeds and spouting seeds were induced organogenesis on different induction media containing TDZ, BA. The regenerated plants via organogenesis were obtained and the effects of plant growth regulator and genotypes were analyzed. Multiple shoots were induced from embryonic axes of all genotypes cultured on all induction media containing TDZ. TDZ could induce the organogenesis of embryonic axes of immature seeds in soybean. High efficiency of organogenesis appeared when the embryonic axes were induced on the media containing 0.75mg/L TDZ combined with 2.0mg/L BA. The media contained higher concentration of NAA could increase the efficiency of organogenesis in soybeans. |
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| Keywords: | Glycine max Organogenesis Thidiazuron Embryonic axis |
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