油茶粕中茶皂素纯化方法与抗菌活性研究

对Amberliter XAD-16和DM130大孔树脂纯化油茶皂素的条件进行探索，比较两种树脂纯化的油茶皂素和AB-8大孔树脂纯化产品的纯度，并测定纯化产品与粗提物的抗菌效果。结果表明，在相应的最佳条件下，Amberliter XAD-16纯化所得的茶皂素产品纯度低，为74.5%；DM130大孔树脂处理的高，为 85.6%；AB-8大孔树脂居中，为83.5%。油茶皂素的粗提物和纯化物对供试微生物的抗菌效果一致，纯化物抑制程度略高；二者均对玉米纹枯病菌抑制效果最明显，对白色葡萄球菌、白色念珠菌和腊状芽孢杆菌的抑菌效果次之，对温和气单胞菌、豚鼠气单胞菌、嗜水气单胞菌、变形杆菌的抑制效果不明显，对黑曲霉、米曲霉的生长没有抑制作用。 茶皂素；油茶；大孔树脂；抗菌性

Purification technology and biological activity of theasaponin from camellia cake

Purification technology of theasaponin from Camellia oleifera cake was studied using Amberliter XAD-16 and DM130 macroporous resins.And antimicrobial activity of rough and purified theasaponin extractions were investigated on 10 microbs in plates.Under their respective optimal condition of Amberliter XAD-16 and DM130 resins,the purity of theasaponin from DM130 was the highest(as the purity of 85.6%),that of Amberliter XAD-16 was the lowest(as 74.5%).The purity from AB-8 resin was 83.5%.Purified product and ...