北方地区白菜型冬油菜与春油菜的SSR和InDel遗传多样性分析

为了合理评价北方地区白菜型油菜种质资源,为抗寒育种及组合配制提供依据,从252对标记中筛选出9对SSR和36对In Del标记对感温性、抗寒性、品质等性状差异明显的19份白菜型油菜品种的遗传多样性和亲缘关系进行了分析。结果表明,共检测到95个等位变异,平均每个标记2.15个;有效等位基因变幅为1.05~3.27个,平均为1.70个。Shannon指数的变幅范围在0.121 7~1.269 5间,平均值为0.580 3;多态性信息量PIC变幅范围为0.049 9~0.637 7,平均值为0.308 1。通过NTSYS计算遗传相似系数GS并按加权配对法(UPGMA)聚类,结果表明19份材料的GS变异在0.52~0.86之间。在GS为0.605水平上可将19份材料按两室与多室性划分为I-1和I-2两大类群,I-1类群在GS为0.655水平上可按冬春性分为II-1、II-2两个亚类群。在GS为0.71水平上,可将冬性材料划分为4个小群。聚类结果表明北方地区白菜型油菜的遗传多样性丰富,冬、春性品种间及春性品种间亲缘关系较远,存在较大遗传差异。

Genetic diversity of winter-type and spring-type turnip rape (Brassia rapa L.) in north China by InDel and SSR markers

To evaluate germplasm for winter turnip rape (Brassica rapa L.) breeding on cold resistance in north China, genetic diversity of 19 turnip rape varieties (which were significantly different in temperature sensitivity, cold hardiness and oil quality) were analyzed using 9 SSR and 36 InDel markers selected from 252 markers. Results showed that 95 allelic variations were detected by 45 SSRs and InDels, with an average of 2.15 alleles each marker. Effective alleles varied from 1.05 to 3.27, with an average of 1.70 each. Shannon index ranged from 0.121 7 to 1.269 5, with an average of 0.580 3. Polymorphism information content (PIC) ranged from 0.049 9 to 0.049 9, with an average of 0.308 1. By NTSYS calculation and unweighted pair-group method with arithmetic mean (UPGMA), genetic similarity (GS) of 19 materials varied between 0.52 to 0.86. They were divided into 2 major groups (I-1 and I-2) with multiple ventricular at GS 0.605. Group I-1 was divided into 2 semi-groups (II-1 and II-2) according to their winterness at GS level of 0.655. Among them, winter-type rapes were divided into 4 small groups at GS 0.71. The cluster results showed abundant genetic diversity with large distance between varieties from different geographic origins and cold hardiness in north China.