拟南芥叶片和角果特异性启动子的筛选与分析

通过生物信息学方法和Genevestigator程序,从拟南芥基因组中筛选了1个叶片特异性表达基因ACD6(at4g14400)和2个角果特异性表达基因AT2FP2(at5g57520)、at1g56100,并通过实时定量PCR分析它们在拟南芥不同组织中的表达模式。从TAIR网站获得各基因的上游序列,利用Plant CARE和Bio Prospector预测各基因的启动子序列,命名为Pat4g14400、Pat5g57520和Pat1g56100。构建启动子驱动报告基因gus的植物表达载体,转化拟南芥。转基因拟南芥GUS染色后发现:ACD6主要在叶片中表达,Pat4g14400为叶片特异性启动子;AT2FP2和at1g56100在角果中的表达量比其他组织高数倍,Pat1g56100和Pat5g57520具有角果特异性。

Leaf and silique of specific promoters of Arabidopsis thaliana

 A leaf-specific expressed gene ACD6 and 2 silique-specifically expressed genes (AT2FP2, at1g56100) of Arabidopsis thaliana were obtained from Genevestigator by bioinformatics search. Gene expression patterns were detected employing real-time quantitative PCR. The upstream regions of these genes were obtained from TAIR website, and promoter sequences were predicted by online software PlantCARE and BioProspector. The promoters were named as Pat4g14400, Pat5g57520 and Pat1g56100 respectively. In corresponding transgenic A. thaliana plants, histochemical GUS staining showed that ACD6 was mainly expressed in leaf, Pat4g14400 was leaf-specific. AT2FP2 and at1g56100 expression in silique were both several times higher than in other tissues. It could be concluded that Pat5g57520 and Pat1g56100 were silique-specific.