花生AFLP指纹图谱

ＡＦＬＰ是一种以ＰＣＲ为基础的ＤＮＡ指纹图谱技术。利用ＡＦＬＰ技术对从国际半干旱所（Ｉ－ＣＲＩＳＡＴ）引进的９份花生抗旱品种绘制指纹图谱，通过引物Ｅ—ＡＣＡ和与之匹配的ＭＣＡＧ和Ｍ—ＣＡＴ，在３００～６０００ｂｐ的范围内共获得１５７７条ＡＦＬＰ扩增产物，每个品种有主带和次带至少７１条之多，其中１０条为多态性的带纹。

Fingerprinting in groundnut using AFLP

Amplified fragment length polymorphism(AFLP) is a novel PCR based DNA fingerprinting technique. With the combinations of EACA primer paired with MCAG and MCAT, respectively. Productions of 1577 segregating AFLP amplification in 9 groundnut (Arachis hypogeae L.)varieties from ICRISAT(International Crops Research Institute for the SemiArid Tropics)were identified. AFLP results generated more than 71 polymoiphic bands, contained some major bands and some minor bands, ranging 3006000 base pairs, from one pair of EcoR I primer and Mse I primer and up to 10 polymorphic bands can be detected with a single primer for each varieties on the denatured gel.