防治十字花科作物根肿病的油菜内生细菌分离与鉴定

为探寻对十字花科根肿病有生防作用的菌株，本文从油菜根内组织分离内生细菌，通过根肿菌孢子裂解、盆栽防病试验证明其功能，并经形态学、生理生化和16S RNA基因序列鉴定其分类地位。研究结果表明，从油菜品种KT1004和Y05-84-5-1根部分离出51株内生菌，其中YN201305和YN201310对十字花科根肿病菌有明显裂解和抑制作用。盆栽试验表明，应用浓度107 CFU/mL的菌液，在播种时和播种后间隔5d，浇灌1次，共浇灌4次。45d苗龄调查结果，二者对大白菜品种鸿源83的根肿病防治效果分别达65.12%和77.92%。经鉴定，YN201305为短小芽孢杆菌，YN201310为枯草芽孢杆菌。

Isolation and identification of endophytic bacteria from canola with control effect on clubroot disease of cruciferous crops

To lyze Plasmodiophora brassicae spores and control clubroot disease of cruciferous crops, endophytic bacterial strains were isolated from canola root tissue. The strains were identified by morphological, physiological, biochemical characteristics and 16S RNA gene sequencing. The biocontrol function of the strains was proved through spores lysis and pot experiment. Fifty-one strains were obtained from roots of two canola varieties, KT1004 and Y05-84-5-1. Among the strains, YN201305 and YN201310 had outstanding effects on lysing P. brassicae spores and clubroot- control. In pot experiments, YN201305 and YN201310 were used to drench Chinese cabbage (B. rapa cv Hongyuan 83) after seeding and emergency for 4 times every 6d. At 45-day seedling stage, the bio-control efficiencies of YN201305 and YN201310 were 65.12% and 77.92% respectively. Identification results showed that YN201305 was Bacillus pumilus and YN201310 was B. subtilis respectively.