多年生稻稻瘟病抗性评价

利用长雄野生稻（Oryza longistaminata）地下茎无性繁殖特性培育多年生稻（Perennial Rice, PR）已经成功并开始示范推广。多年生稻表现出一定的稻瘟病抗性,但其所具有的稻瘟病抗性来源尚不清楚。本研究通过田间病情调查、接种鉴定以及抗性基因检测等3种方法,对育成的多年生稻23（PR23）、云大24（PR24）、云大25（PR25）、云大101（PR101）、云大107（PR107）、父本长雄野生稻、母本RD23、（RD23/长雄野生稻）F₁进行稻瘟病抗性评价。结果表明,父本长雄野生稻、（RD23/长雄野生稻）F₁代及5个多年生稻品种（系）表现为高抗稻瘟病,而母本RD23表现高感稻瘟病,推测PR23、PR24、PR25、PR101和PR107这5个多年生稻品种（系）的稻瘟病抗性可能来源于长雄野生稻。其中,PR23、PR25稻瘟病抗性基因可能来自于长雄野生稻的Pi5基因和Pita-2位点,PR24稻瘟病抗性基因可能是来自长雄野生稻的Pita-2位点,PR107稻瘟病抗性基因可能来自于长雄野生稻的Pi5基因和Pish位点;PR101中未检测到本文中涉及到的基因或位点,推测其稻瘟病抗性来自长雄野生稻内未知的稻瘟病抗性基因。本研究结果将为多年生稻稻瘟病抗病育种、品种布局、植保技术制定等提供一定参考。

Evaluation of Rice Blast Resistance in Perennial Rice

Perennial rice has been developed successful by utilizing the rhizome trait of the wild rice species, Oryza longistaminata and demonstrated widely, exhibiting broad-spectrum resistance to rice blast. To understand the reason of resistance to blast of perennial rice, disease investigation in famer's field, inoculation identification and molecular detection of rice blast resistant genes were carried out among nine accessions including five perennial rice varieties （lines） as Perennial Rice 23（PR23）, Yunda24 （PR24）, Yunda25 （PR25）, Yunda101 （PR101）, Yunda107 （PR107）, male parent O. longistaminata, female parent RD23, F₁（RD23/O. longistaminata） and Lijiangxintuanheigu（LTH）. The results showed that all the five perennial rice varieties（lines）, their male parent O. longistaminata and the original F₁（RD23/O. longistaminata） had high resistance to rice blast, while their female parent RD23 showed high susceptibility, which indicated that the rice blast resistance gene of these five perennial rice lines may originated from O. longistaminata. The resistance of PR23 and PR25 might owing to the Pi5 gene and Pita-2 locus from O. longistaminata, and the resistance of PR24 might due to the pita-2 locus from O. longistaminata, while that of PR107 might inherited from O. longistaminata with the Pi5 gene and Pish locus. However, none of the known rice blast resistance genes or loci mentioned in this study were harbored by PR101, speculated that the unknown genes from O. longistaminata inherited by PR101 archived its blast resistance. The results of this study provided a basis and strategy for blast disease resistance breeding, perennial rice varieties distribution and plant protection technology decision in future.