转基因马铃薯PPO、CAT活性变化及同工酶分析

采用生理生化方法对转基因马铃薯纯合四倍体甘单花9号(GD-9-qc)系列的试管苗进行了PPO活性检测、同工酶分析及CAT活性检测和POD同工酶分析。结果表明:反义PPO基因对大多数转基因马铃薯试管苗PPO的活性产生了明显的抑制效果,其中GD-9-qc-10与GD-9-qc-11的PPO活性比对照降低89.06%和83.98%,且相应转基因品系的PPO同工酶也被明显抑制。实验同时发现,转基因马铃薯不同品系中的过氧化氢酶和过氧化物酶的活性也受到不同程度的影响,表现为有的高于对照,有的低于对照;而POD同工酶所示结果与POD活性检测相一致。

Physical Activity of Transgenic Homozygous Triploid of Potatoes

Sixteen transgenic potato plants in vitro of "Gandanhua No.9" were used to test the PPO and CAT activity and to analyze the PPO and POD isozyme. The results showed that the PPO activity of most samples was repressed by anti-sense PPO gene. The PPO activity of "GD-9-qc-10" and "GD-9-qc-11" was only 2.8 and 4.1(0.01 OD·min-1), decreasing by 89.06 and 83.98 percent, respectively, compared with the control. Meanwhile, the PPO isozyme was depressed significantly also in above strains. It also showed that antisense PPO gene not only repressed PPO activity but also put an wide influence on activity of POD and CAT. It is resumed that because of the insertion of anti-sense PPO gene randomly, it may lead to wide changes of physiological activity of transgenic plants.