| 马铃薯种质离体保存技术 |
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| 引用本文： | 岳新丽,湛润生,帅媛媛,陈云.马铃薯种质离体保存技术[J].中国马铃薯,2012(5):257-259. |
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| 作者姓名： | 岳新丽 湛润生 帅媛媛 陈云 |
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| 作者单位： | 山西省农业科学院高寒区作物研究所;大同大学农学与生命科学学院 |
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| 基金项目： | 山西省农业科学院育种工程项目(YYZJC1117) |
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| 摘 要： | 以马铃薯‘晋薯16号’为试验材料,以节间作为外植体,进行了种质资源缓慢生长保存研究。结果表明,以MS+IAA 0.5 mg/L+KT 0.1 mg/L为基本培养基,通过调节蔗糖浓度进行马铃薯种质离体保存,在常温条件下最佳蔗糖浓度为90 g/L;以MS+蔗糖30 g/L+IAA 0.5 mg/L+KT 0.1 mg/L为基本培养基,通过调节多效唑(PP33)3浓度进行马铃薯种质离体保存,在常温条件下最佳PP333浓度为1.0 mg/L,在低温4℃条件下最佳PP333浓度为0.5 mg/L。离体保存后的试管苗转入继代培养基中进行恢复培养,其生长情况与正常继代苗无显著差异。
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| 关 键 词： | 马铃薯 种质 离体保存 |
In vitro Preservation of Potato Germplasm |
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| YUE Xinli,ZHAN Runsheng,SHUAI Yuanyuan,CHEN Yun.In vitro Preservation of Potato Germplasm[J].Chinese Potato,2012(5):257-259. |
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| Authors: | YUE Xinli ZHAN Runsheng SHUAI Yuanyuan CHEN Yun |
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| Institution: | 1(1.Institute of High Latitude and Cold Area Crops,Shanxi Academy of Agricultural Sciences,Datong,Shanxi 037008,China;2.College of Agriculture and Life Science,Shanxi Datong University,Datong,Shanxi 037009,China) |
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| Abstract: | Restricting conservation is a main method of plant germplasm in vitro preservation.Internode slow growth conservation of potato cultivar 'Jinshu 16' was studied in this experiment.The results showed that MS + IAA 0.5 mg/L + KT 0.1 mg/L plus 90 g/L sucrose gave best results at room temperature,while MS + sucrose 30 g/L + IAA 0.5 mg/L+ KT 0.1 mg/L supplemented with Paelobutrazol(PP333) 1.0 mg/L at room temperature and 0.5 mg/L at low temperature(4℃) performed well.Potato regenerated plantlets were the same as the normal subculture plantlets in morphology,when the plantlets were transferred to subculture medium at room temperature after preserved in vitro. |
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| Keywords: | potato germplasm in vitro conservation |
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